Hi everyone,

I tried using fibrinogen and thrombin to construct a 3D cell culture, but I noticed that my blocks are decreasing in size every day. Here are the concentrations I am using: 5 mg/mL fibrinogen + 8 U/mL thrombin, with polymerization for 30 minutes at 37°C.

Has anyone else experienced this problem? If so, how did you solve it?

Thank you!

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