I have been using the RNeasy Plus Mini kit and have tried to homogenize my tissue samples with a handheld rotor stator, but I can't seem to get the hang of using the machine properly without making it make a horrible grinding noise and get extremely warm.
I have a MagNA Lyser machine in my lab I have used for protein extraction. Can I use this to homogenize my samples if I get the green beads for the system?
Thank you in advance!
Hello,
You can use the MagNA Lyser machine to homogenize your tissue samples.
Let's consider:
Getting the green beads for your tissue type.
Ensuring that you optimize the homogenization conditions, such as the speed and duration, based on your specific tissue.
You might need to adjust the protocol slightly compared to your current method with the RNeasy Plus Mini kit.
Good luck ;-)
Zoe Alexis Walker
My suggestion is instead of using a handheld rotor-stator or MagNA Lyser machine. Why do you try grinding the tissue with the pastel and mortar with liquid nitrogen?
It will work, and even you can use the MagNA Lyser machine for tissue homogenization.
Thank you
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