Absorption Spectroscopy

Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of frequency or wavelength, due... | Contact experts in Absorption Spectroscopy to get answers

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Questions related to Absorption Spectroscopy

Do I need to correct the PL spectra further when using cut-off filter(s) at the emission side?

I'm recording PL spectra with a CCD detector by putting a cut off filter in front of it (laser is used as an excitation source). There is an option, already available, for recording the spectra...

07 July 2017 781 4 View

I plotted %T vs wavenumber for FTIR spectrum.

But, the reviewer commented as follows: "It will be useful to compare if the relative intensities are plotted". My spectrum as attached. Do you have any idea how can I answer/plot a new graph?...

07 July 2017 8,524 7 View

Change in the shape of the spectrum, how to quantify?

Hello, I am currently working on assessing the elastin spectrum properties in different tissues and I'd like to compare the elastin spectral signature between tissues to see whether or not there...

07 July 2017 10,124 8 View

What is the different between the words spectroscopy and spectroscope?

What is the different between the words spectroscopy and spectroscope? for example, should we say Raman spectroscope OR Raman spectroscopy?

07 July 2017 2,919 10 View

What is the reason behind the structered shape in emission maxima of some aromatic amino acids in some solvents?

I have observed some dual peak in emission maxima of tyrosine in some polar solvent. I eagerly want to know what is the reason behind this.

07 July 2017 4,396 2 View

Non-classical tryptophan fluorescence quenching

I have a doubt and difficulty interpreting my tryptophan fluorescence graph since the concentrations do not seem to be creating a pattern in the curves. It would be expected that in larger...

07 July 2017 5,655 1 View

Raman spectroscopy of drugs

I have been successful to set up a Raman configuration to get Raman signal of samples with 785 nm laser.But now I need to complete a project with this setup.I am interested in working on drugs...

07 July 2017 2,447 2 View

What is the actual relationship between Lambda max and time in UV-Vis absorbance spectroscopy?

UV-Vis absorbance spectra of an organic compound shows increasing in Lambda max value upon different time interval in a same solvent. There are no observed shifts in Blue and Red wavelengths, but...

07 July 2017 8,111 6 View

What is the reason behind the structered shape in emission maxima of some aromatic amino acids in some solvents?

I have observed some dual peak in emission maxima of tyrosine in some polar solvent. I eagerly want to know what is the reason behind this.

07 July 2017 3,553 2 View

What happens during continuous usage of spectrophotometer?

If a spectrophotometer is used continuously for three days without turning the lamps off in between, how could it affect the readings?

28 June 2017 662 3 View

What to consider a baseline in Raman spectroscopy?

After measuring an oxide with a Raman spectroscope, I got data that generally look like in the file attached (but with large zoom I can see some tiny peaks): When I subtract a straight line, I got...

13 June 2017 1,089 5 View

How to calculate refractive index from Uv visible spectrum?

send the link i.e formula

12 June 2017 6,604 10 View

Is absorption co-efficient synonymous to photo-sensitivity of a semiconductor material especially when it is grown in polycrystalline thin film form?

What could be the possible reason(s) if it is observed that optical absorption of a polycrystalline thin film is increased after annealing, but the photonsensitivity of the material diminished...

08 June 2017 8,449 4 View

What to consider a baseline in Raman spectroscopy?

After measuring an oxide with a Raman spectroscope, I got data that generally look like in the file attached (but with large zoom I can see some tiny peaks): When I subtract a straight line, I got...

06 June 2017 10,051 4 View

Why do the absorbance values for (HRP-TMB-H2O2) reaction mixture decreasing with time instead of showing linear increase as they should ?

While studying kinetics for HRP with TMB as a substrate, my reaction mixture rapidly turns yellow after turning blue for a very short while. Also, the absorbance taken with 30s interval went up...

06 June 2017 9,345 1 View

Is there a formula in computing the amount of drug released in dissolution testing through UV Vis Spectrophotometer?

Dissolution Studies of experimental drug in reference with a standard.

06 June 2017 2,785 2 View

Quantifying UV irradiation of different lamps by UV sensor?

Greetings I am trying to quantify UV irradiation form different UV lamps buy using UV sensor. The problem is the response curve of the sensor is different for different wavelength (as it is often...

06 June 2017 7,449 4 View

Why do absorption peaks in IR-spectrum are very narrow while absorption peaks in UV/VIS spectrum are relativly wide?

if you look at the peak shape in both IR-spectrum and UV/VIS spectrum of any molecules, you may notice two things. In IR spectrum of small molecules, the absorption peaks always tend to be very...

06 June 2017 5,645 13 View

What is the reason of one peak and two peak in an absorbance graph?

Hello Everyone, I have attached two absorbance graph of two different compounds. Can anyone please tell me the reason for one peak and two peaks in an absorbance graph? and multi-peak as...

06 June 2017 6,905 4 View

What is the difference between PL intensity and normalized PL intensity and how it can be calculated?

06 June 2017 1,379 3 View

Is their any animation video of inverse bremsstrahlung process or free free absorption?

Hello,I read some articles related to free free absorption but still it does not clear to me that who it physical happens. I need animation video regard inverse bremsstrahlung process or free free...

06 June 2017 4,038 0 View

In Absorbance spectra initially Absorbance decreases with wavelength but after 700 m it increases. Can anybody suggest me why it is happening?

I am working with cuins2 thin films. In the absorption spectra initially Absorbance decreases with wavelength and after 700 nm it increases. PLEASE give me the reason.

06 June 2017 7,725 12 View

Can I convert reflectance to absorbance?

I used reflectometer to measure the degree of browning of baked goods. I need to change these values to absorbance because I currently use Colorimeter to measure L value. Is it possible to convert...

06 June 2017 10,651 6 View

Can someone help me to figure out the calculation for black carbon that was detect by Smoke Stain Reflectometer on filter paper?

can someone help me to figure out the calculation for black carbon that was detect by Smoke Stain Reflectometer on filter paper? i was collect PM2.5 using quartz filter paper. Then, i need to...

31 May 2017 3,088 1 View

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