Absorption Spectroscopy

Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of frequency or wavelength, due... | Contact experts in Absorption Spectroscopy to get answers

3,196 views 584 posts

Questions related to Absorption Spectroscopy

Please suggest me if there is any way to increase the refractive index of water to 1.46 at 980 nm?

Please suggest me if there is any way to increase the refractive index of water to 1.46 at 980 nm wavelength laser.

01 January 2017 4,505 1 View

Is the spatial mode of a beam important when we split light with a BS or PBS?

I need to control the probability of transmission/reflection with a PBS and a halfwavelength plate. Diode lasers don't usually have a nice beam shape i.e. it is not Gaussian. Does this affect...

01 January 2017 2,415 2 View

How can I convert epsilon to absorbance (intensity) in UV-vis spectra from Gaussian09 to compare with experimental data?

From Beer-Lambert law, the length and concentration of theoretical data is unknown. How can I convert epsilon to intensity?

01 January 2017 4,918 2 View

How can I deal with the transition overlaps in the Neodymium absorption spectrum when I am doing JO calculation?

In the neodymium absorption spectrum, there are several peaks correspond to two energy level (transition overlaps), but to do the Judd-Ofelt parameter calculation I have to choose one certain...

14 December 2016 9,999 1 View

The formula for calculate the other aldehydes ??

i measured Other aldehydes with a wavelength of 455 nm. now i need formulas to calculate???

12 December 2016 8,358 0 View

Holo protein shows higher absorption than that of Apo proteins when checked with simple absorption scan. Can anyone share their inputs?

Absorption spectra, Protein, spectrophotometer, Calcium

12 December 2016 4,633 0 View

How can I deal with the transition overlaps in the Neodymium absorption spectrum when I am doing JO calculation?

In the neodymium absorption spectrum, there are several peaks correspond to two energy level (transition overlaps), but to do the Judd-Ofelt parameter calculation I have to choose one certain...

12 December 2016 4,255 1 View

What could be the reason behind this discontinuity in UV-Vis spectra?

As you can see there are two clear discontinuities in the F(R) vs Energy spectra which is surely not an effect intrinsic to the sample. Spectra was obtained in Diffuse reflectance mode using a...

12 December 2016 1,497 4 View

Im currently trying to analyse the graf of PL spectra. Could you please advise me on how to analyse this?

Studying about Carbon dots now.. Im trying to understand on how the pl spectra provide the information regarding surface area ets. based on the figure, does it means my emission wavelength have...

12 December 2016 6,069 2 View

I measured Other aldehydes with a wavelength of 455 nm. now i need formulas to calculate??

measured other aldehydes in plants.i measured Other aldehydes with a wavelength of 455 nm. now i need formulas to calculate??

12 December 2016 7,622 0 View

Why does absorption of a material and its emission peak at different frequency?

Why does absorption of a material and its emission peak at different frequency? why does emission spectrum red-shift relative to absorption spectrum?

12 December 2016 9,422 0 View

How to calculate the slope of a line that resembles the exponential decay?

Iam measuring the absorption of substrate in a enzymatic reaction to find out the kinetic parameters Km and Vmax. Plot of the curve (absorbance vs time) obtained in a exponential decay manner. To...

12 December 2016 8,907 3 View

I want to know which absorbtion coefficent i should take to calculate unknown concentration if i got 2 plot for one individual nanoparticle.

I have done UV spectrometer to calculate the unknown concentration of 2 mixture compound. As through lambert equation first I have to know the absorbance coefficient of individual compound . I had...

12 December 2016 959 5 View

SPF value determination using absorption spectra values from 290 to 400 nm?

Hi all, I have come across with few SPF determination protocols using absorption values from 290 to 320 nm, which actually won't give the final SPF value for UVA compounds, so I am interested to...

12 December 2016 729 1 View

Is there any suggestion about the absorption curve of human body to infrared light?

Recently,I am studying on the influences about IR light to human beings. Except the absorption curve about human body to IR light,any related information is well and needed.

12 December 2016 4,401 3 View

What could be the reason behind this discontinuity in UV-Vis spectra?

As you can see there are two clear discontinuities in the F(R) vs Energy spectra which is surely not an effect intrinsic to the sample. Spectra was obtained in Diffuse reflectance mode using a...

10 December 2016 7,181 3 View

Im currently trying to analyse the graf of PL spectra. Could you please advise me on how to analyse this?

Studying about Carbon dots now.. Im trying to understand on how the pl spectra provide the information regarding surface area ets. based on the figure, does it means my emission wavelength have...

08 December 2016 2,295 2 View

How to calculate the slope of a line that resembles the exponential decay?

Iam measuring the absorption of substrate in a enzymatic reaction to find out the kinetic parameters Km and Vmax. Plot of the curve (absorbance vs time) obtained in a exponential decay manner. To...

05 December 2016 7,370 3 View

How can I assign the electronic transition type to each absorption band in UV-VIS spectrum?

I work with ruthenium acetylacetonate and I need to know which absorption band corresponds to which transition. I record the spectrum in water and I get 3 main bands with max absorbance at 270,...

11 November 2016 5,437 4 View

Is there any effective method to eliminate fluorescence impurities on the coverslip?

I find my coverslip has autofluorescence. I tried UV light irradiation, KOH, piranha solution and two types glasses, but they all did not work. Now, what else can I try? Thank you.

11 November 2016 1,416 2 View

How to calculate 1) Amount of drug released and 2) Cumulative percentage release (%)?

How to calculate 1) Amount of drug released and 2) Cumulative percentage release (%). The amount of drug conjugated particles in dialysis bag is 5 ml, Bath volume is 150 ml and sample withdrawn is...

11 November 2016 7,861 11 View

Why there is no absorbance peak for Hydrophobic Doxorubicin?

I am trying to encapsulate hydrophobic doxorubicin in liposme, i can not see any absorbance peak for DOX, but there is a very sharp fluorescence peak of DOX,that indicate that DOX is encapsulated...

11 November 2016 1,634 7 View

Hello All, Does anyone has experience calculating depth on samples through photoacoustic FTIR data? Any hint would be welcomed?

I know about the FTIR modulation frequencies but I am having difficulties getting the relationship right so as to get a value of depth of the sample under analysis? Is there any question somewhere...

27 October 2016 3,141 2 View

Please suggest me a chemical liquid that has very good optical absorbance for the laser of wavelength 1000 nm.

I am using 1000 nm laser in my research and I am searching for a chemical liquid that can absord maximum light from the laser of 1000 nm, in order to verify the quality of my experimental procedure.

10 October 2016 7,081 4 View

Badges
Science method