Absorption Spectroscopy

Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of frequency or wavelength, due... | Contact experts in Absorption Spectroscopy to get answers

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Questions related to Absorption Spectroscopy

What can cause lower absorbance readings from center of 96-well plate following MTT?

I have been running drug treatment assays on cell lines in 96-well plates. Following 72 hours incubation, I perform a MTT assay to assess cell viability following the treatment. I am frequently...

15 May 2018 8,860 4 View

How to synchronise detector (ccd) with mechanical chopper to measure absorbance change (and hence spectra) in Transient absorption spectroscopy ?

1. How to detect change in absorbance (delta A) spectra in transient absorption spectroscopy using CCD, mechanical chopper? 2. How to measure I(unpumped) and I(pumped) both at different...

02 April 2018 5,190 4 View

TD-DFT for phosphorescent emission?

Dear members, Recently, I have calculated the emission energy from T1--> S0 state of molecule A. First I optimize the structure in triplet state multiplicity (0 3). Then I calculate single...

27 February 2018 3,038 3 View

Why is λ excitation of a fluorescent compound different from its λ absorption ?

Zinc-tetrakis(4-N-methyl pyridyl)porphyrin has an absorption soret band at λ=437 nm whereas λexcitation=458 nm. ( λemission=640 nm). How can we explain this difference??

22 February 2018 2,105 12 View

How do I set up a table top temperature control unit for application in transient absorption spectroscopy?

I want to do temperature dependent Transient absorption speectroscopy where in a two pulse technique we detect the transmitted probe. Can you suggest a temperature control set up for this....

05 February 2018 7,943 3 View

How instrumental parameters affect the sensitivity in measuring concentration from absorbance value?

We can determine concentration from absorbance at a particular wavelength as: Absorbance= (absorption co-efficient)x (concentration)x (path length)= - log [Isample/Iblank]. If we measure two...

11 January 2018 1,840 3 View

What is the best way to prepare AuNPs/water nanofluid from gold colloid suspended in PBS?

Purchased product: 25 mL Pack Size 15 nm diameter 1 Optical density Stabilized suspension in 0.1 mM phosphate buffered saline 1.64e12 particle/mL assumed as spherical Required 10 mL of 0.05 mM...

03 December 2017 7,938 4 View

Quercetin conjugation to PEG-NH2?

How the NH2 group of PEG form bond with Quercetin and at what site it will form bond? if two different solvents Water and DMSO have been used at neutral pH.

09 November 2017 2,599 1 View

What are the books/papers that clearly explains the CN stretching vibrations in primary, secondary and tertiary amines?

13 October 2017 1,599 3 View

Which form of Kubelka-Munk function should I used to calculate band gap of powder sample?

There are many from of Kubelka-Munk function I have found in literature: 1. F= (1-R)2/2R 2. F= (1-R2)/2R 3. F(hv)= (1-R(hv))2/2R(hv) Again to calculate direct band gap some used (Fhv)2 where some...

11 October 2017 2,764 5 View

How do I determine if an intramolecular proton transfer is likely to happen in Gaussian simulation?

I have an azo dye, which I suspect it may form intramolecular hydrogen bond after photoisomerization. The intramolecular hydrogen bond may have faciliated the proton transfer. After...

04 October 2017 2,567 7 View

How do I calculate the absorbance for BBCEAS-like systems?

I am working on the calibration of a BBCEAS instrument to determine unknown trace concentrations of NO2. For this purpose I have a gas tank with a known concentration to calibrate my BBCEAS...

16 September 2017 6,597 1 View

Dose Optical Transition (Excitation) from Spin up electronic band to Spin down electronic band is possible?

DearAllI have attache 3 figures of band structure of a Ferro-magnetic material. The difference between VBM-CBM band gap is 1.10 where VBM is for spin down (alpha) electron and CBM for spin up...

14 September 2017 2,928 8 View

How to fit proper gaussians to TIPS pentacene absorption spectrum?

The graph attached is steady state absorption spectrum of dilute TIPS pentacene dissolved in chloroform.(black curve) As the graph indicate, gaussians (green curve) can't fit the vibration...

23 August 2017 474 1 View

Why most papers crosslink GelMA/Irgacure 2959 at 365 nm?

Hi guys, I am trying to understand why most papers use UV light at 365 nm for crosslinking of Gelatin Methacryloyl/Irgacure 2959 solution. I cannot find any UV Vis spectrum that shows me that 365...

07 August 2017 2,305 9 View

How can I lock laser at half power point of scanning fabry perot etalon?

I am trying to lock laser at half transmission point of scanning fabry perot etalon (SA200 ) using electrical feedback to laser driver current source.How can I achieve it. my experiment consists...

07 August 2017 4,730 3 View

What should be the path length when doing enzyme assays in 96 well-microtiter plates?

Hello Everyone, Can someone please tell me path length to be used in the calculations when 96 well plate is used for assaying the activity of enzymes! I assume we dont use 1cm path length as in...

18 July 2017 6,160 7 View

How to measure the glucose sensitivity in water-glucose sample using UV-VIS-NIR Spectrophotometer?

How to measure the glucose sensitivity in water-glucose sample using UV-VIS-NIR Spectrophotometer? At what wavelength, there is a possibility to detect the sensitivity for different glucose...

17 July 2017 3,234 3 View

Change in the shape of the spectrum, how to quantify?

Hello, I am currently working on assessing the elastin spectrum properties in different tissues and I'd like to compare the elastin spectral signature between tissues to see whether or not there...

14 July 2017 7,660 6 View

What is the different between the words spectroscopy and spectroscope?

What is the different between the words spectroscopy and spectroscope? for example, should we say Raman spectroscope OR Raman spectroscopy?

10 July 2017 2,466 9 View

What kind of interaction happend between fuchsine basic and DNA

what the type of binding of F.B with DNA (by groove or other) beside I want know the extension coefficient for Fuchsine basic and in which buffer because I try to determine the extinction...

07 July 2017 8,489 0 View

What should be the path length when doing enzyme assays in 96 well-microtiter plates?

Hello Everyone, Can someone please tell me path length to be used in the calculations when 96 well plate is used for assaying the activity of enzymes! I assume we dont use 1cm path length as in...

07 July 2017 9,845 9 View

Why the UV-vis spectra of 3 chloro phenol changes

we studied the photocatalytic degradation of 3-CP under UV light. it was surprising that its UV-Vis spectra have changed. what is this reason? Please help me. in the below spectra black line is...

07 July 2017 3,986 7 View

How to measure the glucose sensitivity in water-glucose sample using UV-VIS-NIR Spectrophotometer?

How to measure the glucose sensitivity in water-glucose sample using UV-VIS-NIR Spectrophotometer? At what wavelength, there is a possibility to detect the sensitivity for different glucose...

07 July 2017 6,697 3 View

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