Absorption Spectroscopy

Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of frequency or wavelength, due... | Contact experts in Absorption Spectroscopy to get answers

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Questions related to Absorption Spectroscopy

Hello All, Does anyone has experience calculating depth on samples through photoacoustic FTIR data? Any hint would be welcomed?

I know about the FTIR modulation frequencies but I am having difficulties getting the relationship right so as to get a value of depth of the sample under analysis? Is there any question somewhere...

10 October 2016 3,262 2 View

Can we determine the concentration of metal nanoparticles from their UV absorption spectrum?

I wanted to learn whether it's possible to determine the concentration of synthesized metal nanoparticles from their UV-absorption spectrum?

10 October 2016 7,841 3 View

About measurement of absorbance in ABTS.+ decolorization assay ?

i have a lot of referencies about ABTS.+ decolorization assay but i have a doubt about measurement of absorbance of control : some authors such Katalinic et al. (2005) (attached pdf file) say that...

10 October 2016 9,142 0 View

Are there any instrument different than spectrometer used to measure absorbance ?

Dear all, I want to do calibration curve for my project ,but unfortunately all spectrometers in the university the wavelength is form 300 nm and above. So I am researching for another instrument...

08 October 2016 9,766 3 View

How to dissociate PLB pentamer into monomers?

Hello everyone, I have a colleague that is running a gel electrophoresis and immunoblotting phospholamban, p-phospholamban-Thr17 and p-phospholamban-Ser16 in rat cardiac muscle. She is loading 5...

09 September 2016 3,799 2 View

Dielectric function and absorption spectra in VASP?

I am researching on solar cell system and very new in this field. I came to know that the imaginary part of dielectric function somehow relates to the absorption spectra of a material. I read some...

19 August 2016 6,497 4 View

What is the detection limit for a species (in time) of absorbance spectroscopy?

How long must a species last for, for it be detectable through typical absorbance spectroscopy. Say I have an enzyme with a turnover number of 300. Each reaction would take only 3.3 milli seconds....

11 August 2016 4,737 3 View

What is the detection limit for a species (in time) of absorbance spectroscopy?

How long must a species last for, for it be detectable through typical absorbance spectroscopy. Say I have an enzyme with a turnover number of 300. Each reaction would take only 3.3 milli seconds....

08 August 2016 1,153 4 View

How to neutralize/remove the source's spectra to obtain absorption spectra?

We have made a CCD detector based spectrometer but are facing a problem while taking the absorption spectra. We are using tungsten lamp as source and stored its spectrum as Yref. Next we placed a...

08 August 2016 5,096 10 View

Why does my sample have a lower absorbance than my blank?

I've been working determining the possible excitation and emission wavelengths for a drug that I've been studying in lab. I planned on measuring the absorbance (200-800 nm), using the maximum as...

08 August 2016 2,067 3 View

How can I correct baseline shift in an in vivo absorption spectrum?

Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....

12 July 2016 2,261 6 View

How can I calibrate a UV-Vis spectrophotometer?

I came to understand that I am only doing a check of absorbance instead of internal calibration. Anyone with useful information should please help.

08 July 2016 6,489 10 View

have anyone researched about "Tunable diode laser absorption spectroscopy For Monitoring co2, CO, NO , ... ?

I need to design and construct a Tunable diode laser absorption spectroscopy For Monitoring Co2, CO, NO , ... . if somebody has worked on this subject, please contact me.

07 July 2016 1,754 3 View

What is the single-phonon region?

in IR absorbtion or raman

07 July 2016 6,588 0 View

UV-vis spectra of Cu-CuO Nps?

Dear Madam/Sir, Can you please clear my doubt about UV-Vis spectra of Cu-CuO Nps. I received two peaks, What does it indicate, what is the exact peak range for Cu and CuO Nps. please find the...

07 July 2016 7,094 4 View

How can I correct baseline shift in an in vivo absorption spectrum?

Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....

07 July 2016 7,485 7 View

How to interpret negative absorptions in ATR-FTIR spectra?

I am wondering why do I get negatve absorptions when taking an ATR-FTIR scan of epoxy resins. Please see the file attached. I know that the negative peak at ~2400 cm-1 is attributed to CO2 and can...

07 July 2016 9,875 18 View

How to limit LDH Oxidation during the extraction process?

Hello, I am doing research on the glycolytic pathway and have been getting unreliable results for my lactate dehydrogenase assays, due to LDH being subject to oxidation. Does anybody have any...

07 July 2016 6,375 1 View

How can I calibrate a UV-Vis spectrophotometer?

I came to understand that I am only doing a check of absorbance instead of internal calibration. Anyone with useful information should please help.

07 July 2016 7,694 11 View

How to limit LDH Oxidation during the extraction process?

Hello, I am doing research on the glycolytic pathway and have been getting unreliable results for my lactate dehydrogenase assays, due to LDH being subject to oxidation. Does anybody have any...

07 July 2016 909 1 View

How can I deal with high slope ratio (SR) values?

Hi everyone.In the context of this project (https://www.researchgate.net/project/Large-scale-variation-of-doc-cdom-from-lakes-to-oceans), we are calculating CDOM metrics on a large number of...

20 June 2016 5,980 3 View

Is there any effect of acid on pi to pi* or n to pi* transition of solvated cluster ?

In condense phase if cluster has been formed. After that with addition of acid what type of change will be there?

18 June 2016 3,375 2 View

Can any one suggest AAS facility for analysis of heavy metal in plant samples?

institute with Atomic absorption spectrometer facility for analysis of heavy metal like Cu, Cd, Zn, Mn, Ni, Fe,Co, Pb and Hg in plant samples with minimal charges or free for research students...

13 June 2016 9,703 7 View

Where can i find a graph showing absorption spectrum of indole-3-acetic acid at visible light wavelenghts?

I'm looking for a graph showing absorption spectrum of indole-3-acetic acid in the wavelengths of visible light, but I was only able to find its spectrum in the UV. Is there a form of online...

10 June 2016 9,874 4 View

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