Absorption Spectroscopy

Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of frequency or wavelength, due... | Contact experts in Absorption Spectroscopy to get answers

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Questions related to Absorption Spectroscopy

What is the correction factor for resonance light scattering?

what is the correction factor for resonance light scattering instrument ?when we use conventional fluorescence spectrophotometer for RLS spectrum in dye aggregates...

30 May 2017 4,020 1 View

Can we estimate fluorescence and absorption profiles from molecular structures?

The absorption profiles are determined by the shape of the aromatic moeity of the molecule but is there any study or software on estimation of absorbance of fluorescence profile based on molecular...

19 May 2017 8,351 5 View

I get negative value of absorbance of dye solution what can i do?

i add substance with ph = 11 to study the effect of ph on adsorption of dye on surface but i get negative value of absorbance using uv spectrophotometer after shaking 1 hour

16 May 2017 3,307 3 View

How can I measure the reflectance and absorbance of a thin film solar cell?

For example, if I wish to add a distributed Bragg Reflector (DBR) in thin film c-Si or CIGS solar cell, how can I measure the reflectance and absorbance of the film with respect to different...

11 May 2017 9,365 6 View

Does varying the excitation wavelength affect the emission wavelength?

I excited a fused aromatic conjugated naphthalene-based system from 240-360 nm and the emission bank was continuously red shifted from 500-800 nm. The absorption maximum is around 360 nm.

10 May 2017 6,455 4 View

Do visible light degrade Rhodamine B without semiconductor?

Do the UV-VIS absorption spectra increase or decrease? (after exposing RhB under visible light)

08 May 2017 6,868 19 View

Hello, Is anyone can help me to calcilate UV-Vis spectra from TDDFT

Hello, Is anyone can help me with the simulation of the UV-Vis spectra from TDDFT, how can I obtain absorbance from oscillator strenght and excitation energy. Thank you

05 May 2017 4,715 3 View

Can someone help me to figure out the calculation for black carbon that was detect by Smoke Stain Reflectometer on filter paper?

can someone help me to figure out the calculation for black carbon that was detect by Smoke Stain Reflectometer on filter paper? i was collect PM2.5 using quartz filter paper. Then, i need to...

05 May 2017 2,497 1 View

What is the correction factor for resonance light scattering?

what is the correction factor for resonance light scattering instrument ?when we use conventional fluorescence spectrophotometer for RLS spectrum in dye aggregates...

05 May 2017 2,350 1 View

Can any one share an experience of any polymer in which the pore size can be photo modulated?

Photomodulation by specific wavelength of light

05 May 2017 9,341 2 View

How to calculate the refractive index, second order dielectric constant for powder samples?

I have recorded solid UV for my powder samples and calculated the band gap. Will it be possible to calculate the refractive index and second order dielectric constant for the same?

05 May 2017 4,496 12 View

Why the oxide ratio of nanoparticles fluctuates in different liquid media?

I have prepared tin and tin oxide nanoparticles using laser ablation method. I have fixed the time and wavelength but vary the laser energy and liquid media ( acetone, ethanol and methanol). The...

05 May 2017 5,189 4 View

How does surface roughness affect the quality of ATR-FTIR spectra, especially for polymeric substrates?

My concern is that a highly rough surface would introduce points of non-contact between the sample and ATR crystal, which may mean the background would not be properly subtracted out. %T may be...

05 May 2017 762 5 View

What is the Meaning of Transmittance % at particular 200 nm wavelength in UV Spectrophotometer

Dear all I wish to ask that meaning of Transmittance % (Example 20 %) at 200 nm wavelength in UV Spectrophotometer can be judged as that " 20 % Particles in our solution are at 200 nm". Is my...

05 May 2017 3,116 3 View

Does anyone know what are the compounds involved in the water samples getting high peaks at 292 nm and 356 nm wavelengths using spectrophotometer?

I measured water samples from 200 to 800 nm using spectrophotometer.

05 May 2017 6,004 4 View

Why Variation in UV spectrometer reading of same sample stock

Lets say I prepare 50 ml sample and took 10 ml for UV analysis at 650,660,670 nm (Abs. recorded i.e 0.5,1.5,1.3 respectively). after short interval similarly when another 10 ml from sample was...

05 May 2017 2,286 5 View

Good nigth. Does anybody have some absorption spectrum of C60 and of some derivatives of it?

I'm an undergraduate chemistry student and I work whit the regiochemistry of C60 fullerene. One of my thecniques of characterization was UV-vis spectrophotometry and fluorescence...

05 May 2017 5,837 1 View

Can we estimate fluorescence and absorption profiles from molecular structures?

The absorption profiles are determined by the shape of the aromatic moeity of the molecule but is there any study or software on estimation of absorbance of fluorescence profile based on molecular...

05 May 2017 5,460 6 View

How can I understand the basics of 2-D electronic spectroscopy?

Especially how do I interpret 2-D electronic spectra? What do the absorption and emission axes signify?

05 May 2017 3,158 2 View

Which model to use to estimate temp change in a material due to light absorption?

I know absorption spectrum of my material and power of the incoming light (both vs. wavelength). Is there any model/equations which can be used to predict the temperature change using these known...

05 May 2017 4,773 4 View

What is the actual information we are getting by subjecting synthesised nanoparticles into UV Visible spectroscopy..?

What is the information we are getting from UV Visible spectroscopy

05 May 2017 6,374 3 View

How can I interpret the UV.visible spectroscopy ?

during spectrophotometer analyzing for a solution contains nanoparticles and protein I get 2 peaks at 420 and 280 nm . How can interpret these peaks ?

05 May 2017 1,731 8 View

Can same protein have different unfolding mechanism with different denaturant like urea and Gdmcl ?

In my case, with urea fluorescenece intensity increases with red shift and with GdmCl fluorescence intensity decreases with red shift.

05 May 2017 698 6 View

Does varying the excitation wavelength affect the emission wavelength?

I excited a fused aromatic conjugated naphthalene-based system from 240-360 nm and the emission bank was continuously red shifted from 500-800 nm. The absorption maximum is around 360 nm.

05 May 2017 3,592 3 View

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