How to measure the glucose sensitivity in water-glucose sample using UV-VIS-NIR Spectrophotometer? At what wavelength, there is a possibility to detect the sensitivity for different glucose concentration?
Not really! Glucose has a very 'poor' UV spectrum though it does absorb below 210 nm with 'everything else'. It has NO visible spectrum unless you add a sulfuric acid reagent. It does have an IR spectrum, but weak radiation means a weak response.
Normally, in environmental samples it is detected online by its 'refractive index'.
Sugars in aqueous solutions are often quantified using NIR spectra and PLS calibration. Bruce is right, there is no way for specific detection on UV-VIS without chemical treatment. The chemometric approach gives ways better results than calibration at single wavelengths in NIR spectra. cf. https://doi.org/10.1016/S0008-6215(01)00244-0
- Badges
- Science method
- Similar topics
- Methodology
- Absorption Spectroscopy