Who can suggest a fast positive control for beta-Gal staining ?

Hello, I want to stain MCF7 cells treated and untreated with beta- Gal. My treatment lasts for 12 days, then I harvest the cells. Does someone know how I could treat MCF7 cells to get a...

08 September 2018 9,266 10 View

How could I visualize senescence in vivo ?

I would like to see whether my in vivo treatment induces senescence in my tumor or the microenvironment......and was wondering if I could simply stain the surrounding skin (that has been around...

10 November 2017 3,767 3 View

Does Serum derived from the fetus contain hormones?

Hello all, I am working with hormone responsive cancer cells (MCF7, ER positive) and wondering whether I need to use stripped serum or not . In literature, some people either strip their...

09 October 2017 9,921 9 View

WHEN using WHICH enzyme mix to isolate tumor cells from xenograft masses ?

06 July 2017 6,201 2 View

Who's experience with the Comet assay, knows how to save costs especially for use of enzymes ( hOGG1,FPG or EndoIII)?

03 April 2017 4,449 4 View

How many B16 do you inject in mice and how long is incubation time before starting the experiment?

Hello, I've a general question as literature is inconsistant: Who has experience with melanoma in vivo models and can help me? I tried to inject 200 thousand cells s.c w/o Matrigel and started...

01 February 2017 6,377 8 View

Can I mix F2 and F3 generations in 1 big mouse Experiment?

Hello, I need to start a quite big mouse experiment and have right now F1 mice (parents were bought form a company) as breeding couples. They wont give enough mice though to start the experiment...

09 October 2016 7,970 4 View

Who looks at Estrogen Receptor alpha and/or Beta?

Hello, does anyone have a personal suggestion regarding Antibodies for Estrogen Receptor alpha and beta ? There are so many out there and usually I go for Cell Signalling, but the references do...

09 October 2016 5,205 2 View

Orally administered drug at ph 4 in Sodium Lactate. D or L- enantiomer?

I want to use Palbociclib in vivo and if in References is said how they diluted, they give you Lactate Buffer (50mM, pH4) as indication. 1) Do I have to care about DL isomers? 2) Could the pH...

07 August 2016 7,296 4 View

Who has experience with sub cutaneous injection of oil diluted drug in mice (NOD SCID)?

07 August 2016 5,265 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View