Who can suggest a fast positive control for beta-Gal staining ?

Hello, I want to stain MCF7 cells treated and untreated with beta- Gal. My treatment lasts for 12 days, then I harvest the cells. Does someone know how I could treat MCF7 cells to get a...

08 September 2018 9,266 10 View

How could I visualize senescence in vivo ?

I would like to see whether my in vivo treatment induces senescence in my tumor or the microenvironment......and was wondering if I could simply stain the surrounding skin (that has been around...

10 November 2017 3,767 3 View

Does Serum derived from the fetus contain hormones?

Hello all, I am working with hormone responsive cancer cells (MCF7, ER positive) and wondering whether I need to use stripped serum or not . In literature, some people either strip their...

09 October 2017 9,921 9 View

WHEN using WHICH enzyme mix to isolate tumor cells from xenograft masses ?

06 July 2017 6,201 2 View

Who's experience with the Comet assay, knows how to save costs especially for use of enzymes ( hOGG1,FPG or EndoIII)?

03 April 2017 4,449 4 View

How many B16 do you inject in mice and how long is incubation time before starting the experiment?

Hello, I've a general question as literature is inconsistant: Who has experience with melanoma in vivo models and can help me? I tried to inject 200 thousand cells s.c w/o Matrigel and started...

01 February 2017 6,377 8 View

Can I mix F2 and F3 generations in 1 big mouse Experiment?

Hello, I need to start a quite big mouse experiment and have right now F1 mice (parents were bought form a company) as breeding couples. They wont give enough mice though to start the experiment...

09 October 2016 7,970 4 View

Who looks at Estrogen Receptor alpha and/or Beta?

Hello, does anyone have a personal suggestion regarding Antibodies for Estrogen Receptor alpha and beta ? There are so many out there and usually I go for Cell Signalling, but the references do...

09 October 2016 5,205 2 View

Orally administered drug at ph 4 in Sodium Lactate. D or L- enantiomer?

I want to use Palbociclib in vivo and if in References is said how they diluted, they give you Lactate Buffer (50mM, pH4) as indication. 1) Do I have to care about DL isomers? 2) Could the pH...

07 August 2016 7,296 4 View

Who has experience with sub cutaneous injection of oil diluted drug in mice (NOD SCID)?

07 August 2016 5,265 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View