Who can suggest a fast positive control for beta-Gal staining ?

Hello, I want to stain MCF7 cells treated and untreated with beta- Gal. My treatment lasts for 12 days, then I harvest the cells. Does someone know how I could treat MCF7 cells to get a...

08 September 2018 9,266 10 View

How could I visualize senescence in vivo ?

I would like to see whether my in vivo treatment induces senescence in my tumor or the microenvironment......and was wondering if I could simply stain the surrounding skin (that has been around...

10 November 2017 3,767 3 View

Does Serum derived from the fetus contain hormones?

Hello all, I am working with hormone responsive cancer cells (MCF7, ER positive) and wondering whether I need to use stripped serum or not . In literature, some people either strip their...

09 October 2017 9,921 9 View

WHEN using WHICH enzyme mix to isolate tumor cells from xenograft masses ?

06 July 2017 6,201 2 View

Who's experience with the Comet assay, knows how to save costs especially for use of enzymes ( hOGG1,FPG or EndoIII)?

03 April 2017 4,449 4 View

How many B16 do you inject in mice and how long is incubation time before starting the experiment?

Hello, I've a general question as literature is inconsistant: Who has experience with melanoma in vivo models and can help me? I tried to inject 200 thousand cells s.c w/o Matrigel and started...

01 February 2017 6,377 8 View

Can I mix F2 and F3 generations in 1 big mouse Experiment?

Hello, I need to start a quite big mouse experiment and have right now F1 mice (parents were bought form a company) as breeding couples. They wont give enough mice though to start the experiment...

09 October 2016 7,970 4 View

Who looks at Estrogen Receptor alpha and/or Beta?

Hello, does anyone have a personal suggestion regarding Antibodies for Estrogen Receptor alpha and beta ? There are so many out there and usually I go for Cell Signalling, but the references do...

09 October 2016 5,205 2 View

Orally administered drug at ph 4 in Sodium Lactate. D or L- enantiomer?

I want to use Palbociclib in vivo and if in References is said how they diluted, they give you Lactate Buffer (50mM, pH4) as indication. 1) Do I have to care about DL isomers? 2) Could the pH...

07 August 2016 7,296 4 View

Who has experience with sub cutaneous injection of oil diluted drug in mice (NOD SCID)?

07 August 2016 5,265 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View