31 Questions 38 Answers 0 Followers
Questions related from Surendra Nath
Hii colleagues, I have data set for a particular district of telangana state, India. The data is about area particulars - village level for Mango in Adilabad District, Telangana state, India and...
08 March 2020 5,843 5 View
I have heard a lot about the impossibility of detecting sex of gynodioecious papaya by any possible means at the nursery stage. Is it really so? i have tried googling but in vain further...
25 July 2016 4,010 3 View
Friends, I want to perform LC/MS for marigold oleoresin before saponification and after saponification. Most of the available literature pertains to LCMS using APCI source for ionization. But...
20 July 2016 3,666 5 View
Friends, I have gone through ample literature only to get confused in selecting a good wall material for nanoencapsulation. We all know that lutein is highly hydrophobic but most of the food grade...
17 July 2016 4,555 4 View
Friends, I want to study the marigold oleoresin extracted using hexane in LC-MS/MS for confirming lutein esters. Also I would like to run a parallel sample which is saponified (with the esters...
17 July 2016 5,489 5 View
While fixing agricultural experiment design such as CRD, RBD, LSD, SPD etc most of scientists caution that the error degree of freedom should be greater that 12. but can anybody let me know the...
01 May 2016 8,202 9 View
Friends, I have doe antioxidant assays DPPH and Total antioxidant activity (TAA) for extracts of marigold germplasm. After the procedures I have obtained the OD values but now I need to analyze...
04 March 2016 8,699 4 View
Friends, I am from coastal Andhra Pradesh, India and would like to have some plants having both mosquito repellant and aesthetic significance. Also if possible their availablity in Coastal Andhra...
13 December 2015 562 12 View
can anyone please share with me the protocols or procedures and necessary precautions to be taken while preparing the stains and fixatives for studying the microsporogenesis and pollen development?
25 September 2015 2,804 2 View
Friends and fellow scientists, Whenever I and any of lab colleagues are preparing the MS solution for preparing the MS media, we make up the volume to 1L and check the pH (around 3.8-4.1) and when...
13 September 2015 253 7 View
Friends, I have been trying to extract essential oil from leaves and flower calyx portions of marigold but not getting the optimum/ expected GCMS result i.e. the compounds i get are very few and...
02 September 2015 8,915 24 View
The crop of my work is Gossypium hirsutum. Can anyone please suggest me the Physical & chemical mutagens that can induce mitochondrial mutations leading to cytoplasmic male sterility....
28 August 2015 534 2 View
Can anyone please discuss the major difference between ION BEAM IRRADIATION ,NEUTRON BEAM IRRADIATION and GAMMA IRRADIATION? Among these irradiation treatments,which will be more beneficial for...
28 August 2015 127 6 View
Friends and scientists, THe image is from my friends chrysanthemum field. the flower (at very few places in the field) is developing white tinge/ bleached colouration at the border of the petals?...
22 August 2015 6,622 4 View
friends, I was studying a paper on lutein purification where they mentioned about using activated silica column 60-120 mesh. So what is silica column and can we prepare it in our labs using silica...
18 August 2015 9,035 6 View
friends, after reading through different articles I have extracted some orange-yellow coloured pigment with THF as solvent from marigold petal powder meal. The residue was obtained after removing...
26 July 2015 7,565 9 View
Friends, I have extracted lutein pigment from marigold petals and now want to encapsulate it in food grade polymers say maltodextrin and guar gum. but have got a problem. Lutein is insoluble in...
23 July 2015 9,051 4 View
I have extracted marigold oleoresin using petroleum ether and have confirmed the presence of lutein but want to quantify it. And also for nanoencapsulation purpose how to seperate pure lutein from...
07 June 2015 7,069 3 View
Certain sprays or coatings are needed to confirm the presence of lutein in marigold oleoresin while working with TLC. Can anybody suggest such sprays (with atomizer) and how to prepare them.
21 May 2015 6,424 6 View
Intellectual Property rights and Breeder Rights in floriculture and landscaping especially in Indian context
16 May 2015 6,763 3 View
Procedure for field experiments, treatments & analysis of data is requested...Thank You
12 April 2015 793 2 View
Can we transform a GMS line into CMS line in Brassica?
12 April 2015 2,661 2 View
Can any one tell me the what is the average content of proline chlorophyll a chlorophyll b total chlorophyll content in bougainvillea and nerium plants under normal conditions as well as stress...
29 March 2015 2,204 2 View
Friends I m working on marigold lutein extraction and quantification can some body please provide me the standard values for the pure lutein so that my values can be interpolated on it and the...
28 March 2015 5,335 5 View
Since lutein standard is expensive, can somebody please specify around how much needs to be purchased, for my marigold analytical purpose.
15 March 2015 4,396 3 View
Please help me in understanding the exact differences between analytical HPLC, semipreparative HPLC and preparative HPLC. I want to extract pure lutein compound from marigold oleoresin and people...
15 March 2015 1,020 6 View
I want to study in vitro mutation in the mentioned bougainvillea cvs...for which I need a standard protocol.....further is LD50 values are fixed for both the cvs....using EMS and gamma...
09 March 2015 7,594 0 View
I'm studying on marigold oleoresin extract which contain lutein. For TLC, HPLC etc. studies I need standard to confirm its prescence. Sigma standard is available but it's too costly. INR...
06 March 2015 10,021 10 View
I have got Coelogyne pods and Knudson C medium for orchid seed germination, but since the seeds are too small and fibrous to handle, feel it extrmely difficult to do and sow uniformly on the...
05 March 2015 1,950 5 View
Please tell me how to screen germplasm for CMS source, what are the means and ways to develop CMS (A) line, B line and C line(Restorer)? And how do you get CMS and restorer lines from the crosses...
13 January 2015 6,978 2 View
I'm doing my research on lutein extraction from marigolds, and further its encapsulation. I have many articles regarding different methods, especially soxhlet, enzymatic extraction and...
25 October 2014 7,757 5 View
