Please tell me how to screen germplasm for CMS source, what are the means and ways to develop CMS (A) line, B line and C line(Restorer)? And how do you get CMS and restorer lines from the crosses of wild species? Please give the details about methodology and analysis.
If I use CHAs to create male sterility can I develop a stable restorer line (Cline)? Please explain in detail.
I have experience of wheat in which CMS source is used as female parent and 5-6 back-crosses are used with recurrent parent (used as male). Recurrent parent is the better agronomic variety in which background you want to transfer CMS. This parent also used as B-line to that specific CMS line.
For restorer line breeding, 3-4 backcrosses followed by selfing for 2-3 cycles is useful.
CHA is not usable for restorer line development as you dont need it.
How to develop a restorer line? Could you please explain the concept or procedure if the source of restoration is not known?
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