Paromik Bhattacharyya

8 Questions 6 Answers 0 Followers

Questions related from Paromik Bhattacharyya

How to extract RNA from pseudobulb of medicinal orchids?

I am trying to extract RNA from the pseudo bulb part of a medicinal terrestrial orchid. although I got high quality RNA from leaf but while trying so from the pseudoibulb or rhizome I am facing...

28 December 2017 2,778 1 View

How to increase cormlet size of Crocus sativus (Saffron) in in vitro cultures?

One of my friend is working on this plant. Wild accesions of crocus sativus are there. however we want to increase its size for commercial exploitation.As reports on in vitro growth is quite less...

09 March 2017 8,170 3 View

Does the use of molecular markers for checking generation wise clonal fidelity not a useful tool?

I want to check the clonal fidelity of a medicinal orchid propagated from the mother plant using axillary branching.Now cross verify the robustness of the protocol I want to use molecular markers...

09 June 2015 1,655 9 View

Which one is more accepted in case of plant tissue culture: expressing PGR concentrations in mg/l or micrmoles?

I am confused regarding this PGR concentration.Till date I have done all my experiments using mg/l method. Many like me has also done the same. But recently I have encountered certain issues where...

02 March 2015 7,091 7 View

Does the ploidy level of a species govern the patterns of gene expression in a plant species?

How is ploidy linked with gene expression of various significant genes such as PAL, troponine reductase, etc?

04 April 2014 6,678 1 View

I want the promoter sequence of Acetyl CoA carboxylase gene of Oryza sativa. Any suggestions how to get Acetyl CoA carboxylase gene of Oryza sativa?

I have searched NCBI for this particular sequence. But I am not getting anything. I think I am making some mistakes in the search. Can anybody please help me?

02 January 2014 962 5 View

Can GENE SCAN analysis using automated DNA sequencer be applied for analysis of SRAP molecular marker data?

I have came across papers where people have used GENE SCAN analysis to analyse AFLP marker datas. This feature is present in all ABI automated DNA sequencers. Can this approach be applied in case...

01 January 2014 799 2 View

After transformation using agarobacterium in legumes I am having problems in rooting. What may be the reason?

I am facing problems with transformation after successful transformation of the plant. But when I am introducing the plants for climatic adaptation the roots are dying. What may be the solution?

04 July 2013 9,133 3 View