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Questions related from Paromik Bhattacharyya
I am trying to extract RNA from the pseudo bulb part of a medicinal terrestrial orchid. although I got high quality RNA from leaf but while trying so from the pseudoibulb or rhizome I am facing...
28 December 2017 2,889 1 View
I am trying to reintroduce micropropagated terrestrial orchids mentioned above. Which substratum is best for growing them?
08 September 2017 9,336 3 View
While hardening/acclimatizing micropropagaed orchids it is seen that lowering of the te,perature gives the best result. What might be the best suited physiological as well as molecular reason...
27 July 2017 4,230 3 View
One of my friend is working on this plant. Wild accesions of crocus sativus are there. however we want to increase its size for commercial exploitation.As reports on in vitro growth is quite less...
09 March 2017 8,304 3 View
I am getting around 7% variability in callus mediated plants and about 3% variability in direct shoot organogenesis derived plants.Now is there any epigenetic angle behind this result? I have seen...
12 September 2015 5,659 3 View
I am trying to judge how, where and why the cryptic genetic variability arises in the course of propagation pathway using molecular markers. While doing the AMOVA I could find that there exists a...
15 June 2015 8,688 7 View
I want to check the clonal fidelity of a medicinal orchid propagated from the mother plant using axillary branching.Now cross verify the robustness of the protocol I want to use molecular markers...
09 June 2015 1,782 9 View
I am studying the flower initiation and the genetic reasons behind it in orchids.I have noticed certain dendrobes like dendrobium aphyllum specially leaves are totally gone when flowers bloom....
07 April 2015 6,094 1 View
My present research is focusing on the evolution of plant secondary metabolites within a particular group of plants of medicinal importance. I have gone through a review of Wink which I am...
23 March 2015 8,827 6 View
I am confused regarding this PGR concentration.Till date I have done all my experiments using mg/l method. Many like me has also done the same. But recently I have encountered certain issues where...
02 March 2015 7,136 7 View
I had communicated a paper to a peer reviewed journal of Elsevier where one of the reviewer has made a statement "The plant growth regulators (PGRs) were evaluated using 'mg/l' which make...
01 March 2015 8,367 20 View
I had carried out a micropropagation work of Dendrobium nobile using in vitro raised explants nicely before and from the excised explants shoots proliferated nicely,. But when I tried the same...
16 February 2015 2,753 9 View
I want to study the evolution of plant secondary metabolite evolution in medicinally important plant taxa. Recently I came across a paper which I am attaching at the end of this mail where the...
07 October 2014 4,384 1 View
I have successfully acclimatized various species of tissue culture raised Dendrobiums. Although they are thriving well in greenhouse/growth chamber conditions (almost 1 year) flowering is not...
30 September 2014 9,039 5 View
I just now came across a paper where the authors have barcoded the genus cymbopogon using ISSR markers. However, how they have performed this barcoding analysis with binary data is not clarified...
08 September 2014 4,076 15 View
I have seen papers on DNA bar coding, where some are clamming species approach is more acceptable, again some are claiming genus specific approach is more acceptable. I want have a view which will...
02 August 2014 5,546 7 View
I want to study in vitro flowering in orchids. For that I want to know which is the best possible PGR combination
12 July 2014 9,742 5 View
I am following a research paper which is published from our research group.I am enclosing the paper with the question. I want improvise the data analysis part of the work which I intend to do....
09 July 2014 6,446 6 View
I am working on a plant from the family polygonecae, where i found that the callus derived plantlets are having around 20% of somaclonal variability but the plant is also having significantly...
08 April 2014 2,311 4 View
How is ploidy linked with gene expression of various significant genes such as PAL, troponine reductase, etc?
04 April 2014 6,729 1 View
I had done a previous study on D. nobile using SCoT markers to study their natural genetic variations. There I found a higher band number, but while I have used the same primers in case of...
28 March 2014 8,898 0 View
I want to relate antioxidant activity which I have measured using FRAP, DPPH and ABTS assay with that of genetic diversity datas generated by various molecular markers. Can anybody please help me...
14 March 2014 2,370 2 View
Suppose, I germinate some seeds/ induce PLBs from an orchid genera. Now, suppose I culture and raise plantlets from the first round of culture. Then, again using the plantlets from that culture...
26 February 2014 6,705 1 View
I have a confusion in calculating the amount. I took 100mg of dried sample and dissolved it in 100ml of solvents like methanol/chloroform etc. I filtered the solvent after 48 hours of incubation...
23 January 2014 2,525 4 View
I want to relate geographical distance to biochemical parameters (variation in chemical content) of a plant. I came across papers where they used a Mahalanobis distance matrix to relate these...
06 January 2014 7,691 4 View
I have searched NCBI for this particular sequence. But I am not getting anything. I think I am making some mistakes in the search. Can anybody please help me?
02 January 2014 1,018 5 View
I have came across papers where people have used GENE SCAN analysis to analyse AFLP marker datas. This feature is present in all ABI automated DNA sequencers. Can this approach be applied in case...
01 January 2014 852 2 View
I had germinated the orchid seeds and got plantlets. Then I have used the raised plantlets as explant source. I have found that the plantlets which were derived from these explants as source are...
04 October 2013 9,257 2 View
I want to establish cultures of certain dendrobes from ex vivo source.Can you please suggest the PGRs whose combinations/individuals are ideal?
10 September 2013 2,207 7 View
Generally, its thought that seed raised plants of a crosspolinated species have high genetic variability. To crosscheck it , I carried out an experiment where I found lesser degree of variability,...
26 August 2013 420 1 View
I have seen in my experiments (orchids) that the rate of variability is much higher when i am germinating a orchid pod and then geeting the plantlets and afterwards extracting DNA from the raised...
02 August 2013 4,321 1 View
The Start Codon Targetted Polymorphism (SCoT) marker has recently become the marker of choice in the case of genetic diversity studies. Can anybody please suggest why it has a greater acceptance...
14 July 2013 6,173 17 View
I am facing problems with transformation after successful transformation of the plant. But when I am introducing the plants for climatic adaptation the roots are dying. What may be the solution?
04 July 2013 9,181 3 View
I have compared clones raised from both coconut water and synthetic PGRs. When I checked the fidelity of the hardened plants where I used coconut water as the additive it gave a lesser degree of...
04 July 2013 5,034 3 View
Plant tissue culture protocols
25 June 2013 4,357 7 View
The population which reveals highest diversity can I go for its sustainable conservation?
23 April 2013 4,648 1 View
Invitro flowering of ornamental and horticulturally important plants.
04 April 2013 4,446 0 View
