Generally, its thought that seed raised plants of a crosspolinated species have high genetic variability. To crosscheck it , I carried out an experiment where I found lesser degree of variability, ranging from 2-8%. I extracted DNA from hardened and acclamatised plantlets of a crosspolinated orchid species and carried out its stability analysis using RAPD and ISSR markers- I found that the percentage ranged from 2% to 5%. What can be the possible reason? Does PGR have a direct implication on its fidelity? I am attaching a paper which i followed while doing this analysis.
Variability is possible in cross pollinated species, but what if both parents are genetically not distinctive from each other, like crossing between clonal plants which are very similar to each other?
The technique you are using to check the fidelity for example the markers and the location where they flank in genomic DNA, you are using are efficient enough to bring out the variations?
Plant Growth Regulators (if this what your abbreviation stands for) in tissue culture may not change the genetic make up of the plant and it causes only physiological and morphological changes.
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