Invitro flowering of ornamental and horticulturally important plants.
I need to analyse proximate composition in whole fish and antioxidant enzyme analysis. What is the protocol to store these samples for maximum efficiency in the results. I have -80 degree C...
05 June 2024 1,372 6 View
When we started the experiment at first at 1ppm all the fishes died within 12 hours. repeated the experiments with lower concentrations starting from 0.05 ppm and found a very good response to the...
02 March 2024 3,097 8 View
There are so many papers available to solve initial value problem by using Fuzzy Laplace Transform method. But need solution for the boundary value problem by using the same method.
29 November 2023 9,727 2 View
For a non-normally distributed small sample (N
20 November 2023 1,953 4 View
Actually I want to insert hkl values for my peaks in XRD pattern.. hkl values are not coming automatically in x-pert highscore plus software. So i have to do Rietveld Refinement. But when i am...
12 December 2022 8,784 8 View
How should one normalize the answer correctly, after creating a histogram of the separation between the head and tail of a worm?
07 November 2022 6,542 2 View
I am struggling with a Mycobacterium protein (phthalate esterase), which upon overexpression forms inclusion body (maybe) and it is found in the pellet fraction. I have tried cloning the protein...
27 July 2022 2,716 6 View
I have cloned a phthalate hydrolase (~37 kDa) into a pMAL-c2x vector and overexpressed in E.coli BL21(DE3). Induction condition - 28 degrees for 3h with 0.5mM IPTG. This is a Mycolicibacterium...
07 June 2022 6,486 4 View
I have used Lowry method to calculate protein content in fish muscle. Briefly, 5% tissue homogenate is prepared in 0.25 M sucrose. Centrifuged and the supernatant is used for analysis. 0.1 mL of...
31 May 2022 6,205 0 View
I was going though this paper "Extraction of vanadium from high calcium vanadium slag using direct roasting and soda leaching" but finally how could they extract from the leached solution I didn;t...
02 September 2021 4,167 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View