Hi Paromik, for general orchids' in vitro flowering procedures - you can refer to this book. I've also attached a chapter that we wrote that summarize the entire general approach.

In particular, coconut water (young and green ones, freshly open) should be included as a source of cytokinins.

At the National University of Singapore, Prof C. S. Loh is one of the key person responsible for doing very good in vitro flowering of Dendrobium orchids. I will attached the website of his reference paper for you to follow.

Good luck! Best wishes!

http://link.springer.com/article/10.1007%2Fs00299-006-0242-2

Book The Physiology Of Tropical Orchids In Relation To The Industry

Article The Chemical Composition and Biological Properties of Coconu...

Sir,

I one paper i have seen apple juice has been used.  What do you think apple juice is more condusive than coconut water in triggering  floral genes?

Hi Paromik

i got in vitro flowering in large frequency in MS media supplemented with BAP in combination of GA3 on Guizotia abyssinica. GA3 has the ability convert the plant from vegetative to reproductive phase. so it can be use to produce flowering.

Hi Paromik, we have not tried apple juice before and so we cannot give you any advice. The best way is still to use a defined medium formulation (with known cytokinins) to induce in vitro flowering such that your new method will be reproducible. If you cannot achieve in vitro flowering using defined medium for your particular Dendrobium species, this is where the young and green coconuts' water (undefined medium), will be great back-up plan. Alternatively, if you have the budget, you can measure the cytokinins, auxins and gibberellins (using either LCMS or ELISA) of 100 green coconuts, and then freeze these coconut water collected.

Article Analytical methods for cytokinins

Article Simultaneous analysis of different classes of phytohormones ...

Article Analyses of Gibberellins in Coconut (Cocos Nucifera L.) Wate...

I have found that for some plants antigibberelins like CCC really help in flowering in vitro