I have came across papers where people have used GENE SCAN analysis to analyse AFLP marker datas. This feature is present in all ABI automated DNA sequencers. Can this approach be applied in case of other molecular markers?
Of course. Any device which is employing capillary electrophoresis can be used for fragment analysis of PCR-based markers.
Yes you can implement Gene Scan analysis for SRAP markers also provided your primers are labelled with suitable fluorophores.
Hi, I am wondering if someone can direct me to an ion implantation forum My specific query is: I am learning the SRIM software for simulating ion implantation process. I am however, not finding...
05 May 2020 1,301 2 View
I am trying to implement a continuum damage mechanics based constitutive model in Abaqus using the user-defined subroutine UMAT. In that context, I am having hard time finding any available...
22 October 2019 9,426 3 View
Hello! I've been trying siRNA knockdown using RNAiMAX (invtrogen) but I'm not getting any significant knockdown in the T47D and HCC-1954. I'm using forward transfection. The protocol I uses is: 1....
15 March 2018 5,626 2 View
I was making a polymer membrane for hydrogen fuel cell. in some of the papers i found people have done gold sputtering before SEM ?
05 March 2018 1,746 2 View
I am trying to extract RNA from the pseudo bulb part of a medicinal terrestrial orchid. although I got high quality RNA from leaf but while trying so from the pseudoibulb or rhizome I am facing...
27 December 2017 2,716 1 View
I know that doped SiC epitaxial layers on SiC single crystals are available commercially. Is it also possible to buy SiC epilayers with some regions with n-type and some regions with p-type...
26 April 2017 9,583 4 View
One of my friend is working on this plant. Wild accesions of crocus sativus are there. however we want to increase its size for commercial exploitation.As reports on in vitro growth is quite less...
08 March 2017 8,092 3 View
is it possible to have strong spin orbit coupled electronic motion in 3D??
25 October 2016 3,756 4 View
how to study phytochemistry analysis of cyanobacterial extracts. in India are there any institute or company who can analyse the phytochemistry of extracts
20 February 2016 7,015 3 View
What is the best method to get equilibrium interlayer distance of graphite?
14 October 2015 8,227 1 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View