Can GENE SCAN analysis using automated DNA sequencer be applied for analysis of SRAP molecular marker data?

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How to store fish tissue sample for proximate analysis and antioxidant enzyme analysis?

I need to analyse proximate composition in whole fish and antioxidant enzyme analysis. What is the protocol to store these samples for maximum efficiency in the results. I have -80 degree C...

05 June 2024 1,372 6 View

Acute toxicity test is not showing proper results. Want to know what could be the factors?

When we started the experiment at first at 1ppm all the fishes died within 12 hours. repeated the experiments with lower concentrations starting from 0.05 ppm and found a very good response to the...

02 March 2024 3,097 8 View

How to solve Fuzzy Boundary Value Problem by using Fuzzy Laplace Transform Method?

There are so many papers available to solve initial value problem by using Fuzzy Laplace Transform method. But need solution for the boundary value problem by using the same method.

29 November 2023 9,727 2 View

Can interpretations based on non-parametric tests be accepted for non-normally distributed sample in the fields of Experimental/Survey Research?

For a non-normally distributed small sample (N

20 November 2023 1,953 4 View

When i going to do Rietveld Refinement in X-pert highscore plus it is showing negetive intensities in scan data points.. Why it is showing?

Actually I want to insert hkl values for my peaks in XRD pattern.. hkl values are not coming automatically in x-pert highscore plus software. So i have to do Rietveld Refinement. But when i am...

12 December 2022 8,784 8 View

What's a nice starting reference on calculation of off-diagonal spin correlations using the directed loop (SSE) algorithm?

How should one normalize the answer correctly, after creating a histogram of the separation between the head and tail of a worm?

07 November 2022 6,542 2 View

How to achieve denaturation and renaturation of protein from pellet fraction of the cell (BL21 DE3)?

I am struggling with a Mycobacterium protein (phthalate esterase), which upon overexpression forms inclusion body (maybe) and it is found in the pellet fraction. I have tried cloning the protein...

27 July 2022 2,716 6 View

Lost protein (phthalate hydrolase) activity which I could achieve once but in later attempts the protein is not showing function? Please suggest??

I have cloned a phthalate hydrolase (~37 kDa) into a pMAL-c2x vector and overexpressed in E.coli BL21(DE3). Induction condition - 28 degrees for 3h with 0.5mM IPTG. This is a Mycolicibacterium...

07 June 2022 6,486 4 View

How to calculate protein content per gram of tissue sample in Fish Tissue?

I have used Lowry method to calculate protein content in fish muscle. Briefly, 5% tissue homogenate is prepared in 0.25 M sucrose. Centrifuged and the supernatant is used for analysis. 0.1 mL of...

31 May 2022 6,205 0 View

How metal is extracted from a leached solution?

I was going though this paper "Extraction of vanadium from high calcium vanadium slag using direct roasting and soda leaching" but finally how could they extract from the leached solution I didn;t...

02 September 2021 4,167 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View