38 Questions 38 Answers 0 Followers
Questions related from Muhammad Aslam buzdar
Hi All, I am planning to use pMRP9-1 GFP plasmid to study virulence of Pseudomonas. Can any one send me link or detail description of this plasmid as i am unable to find it. or can any suggest me...
02 February 2018 201 1 View
Dear fellows, i need help regrading plasmid curing of my yeast. i am working with klyveromyces sp. my research is cloning and expression of killer toxin protein by this strain. i wana confirm...
10 October 2010 1,120 3 View
hope you will be fine. as you know we had been discussing about my high molecular protein which was killer toxin. i got spectra of that protein i mean i send to company for mass spectraphotometer....
09 September 2010 1,423 1 View
09 September 2010 7,081 1 View
Dear Friends i am cloning some genes responsible for killer toxin proteins. first i also lated my protein and then did SDS PAGE and checked it mw that was about 68kd.then i get its dequence by...
08 August 2010 6,414 2 View
08 August 2010 3,508 7 View
i am new in the field of protein isolation and quantification. i have to do Bradford's protein assay i got G- 250 coomess brillient blue dye form sigma but i don't know how to make its solution...
06 June 2010 4,906 3 View
hi friends i am doing purifiaction of some yeast killer toxin proteins.i have isolated and purify them by anion exchange chromatography.i got one peak of my protein then i did sds page and get...
06 June 2010 2,783 1 View
Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian goats Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian...
05 May 2010 721 1 View
A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton (Gossypium hirsutum L.) by Wan C. Y. and Wilkins T. A.
04 April 2010 1,601 0 View
04 April 2010 2,938 0 View
1"Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor"by K Al-Aidroos - 1978 2-"Molecular structure of the cell wall receptor for killer toxin...
04 April 2010 2,584 3 View
hi friends, i am doing protein test by bradfrod method. i made coomesbrilliant blue reagent G-250 according to standard protocol.but i did not get same colour of that reagnet as it is stated in...
03 March 2010 5,085 1 View
Hi dear friends i need following article if any possible find it for me send it to me as soon as possible thanks. "A new improved method for the assay of endo-beta-1,3-glucanase" by Philpott...
03 March 2010 3,052 1 View
03 March 2010 4,635 1 View
03 March 2010 2,138 0 View
I am doing protein test by bradford method. I made coomesbrilliant blue reagent G-250 according to standard protocol but I did not get te same colour of that reagent as it is stated in protocol(...
03 March 2010 1,799 3 View
03 March 2010 7,500 3 View
i have one question if some one has discovered a new multicellular organism and he wants to do genomic research but nothing is know genetically before what should be the steps/scheme for starting...
01 January 2010 7,853 1 View
hi n any body tell me by how many ways we can develop a physical map of any organism nd also let meknow the their names and linkes too. i have to design a physical map of marine organism. and...
01 January 2010 4,749 0 View
01 January 2010 4,539 12 View
01 January 2010 2,385 0 View
01 January 2010 4,831 0 View
i am new memeber
01 January 2010 7,067 0 View
i wish you happy,full of joy and a wonderful new year 2010 to all of scienctific community Aslam buzdar
12 December 2009 9,602 2 View
12 December 2009 5,377 3 View
i am doing smart race to clone a gene from yeast.i i did every thing alright but getting no results i mean my particular band but smear. i wana check my cDNA either it is good or not therefore i...
12 December 2009 6,196 1 View
i need follwing paper if some one can send it to me i have no access of it.if possible please download and attach to my email Purification and partial characterization of an exo-β-glucanase from...
12 December 2009 6,100 1 View
i am cloning a gene from yeast.frist i designed degenrate primer and get partial gene successfully.thandesigned primer according to know sequence and i tried inverse PCR to get complete gene but...
12 December 2009 9,120 1 View
hi dear all i am new in research feild and doing my PhD in biotechnolgy.i have to isolate some toxin form killer yeast.infact they are protein in nature i have to check their activity i.e its...
12 December 2009 2,393 0 View
12 December 2009 3,333 1 View
12 December 2009 8,261 0 View
12 December 2009 9,998 6 View
12 December 2009 3,885 1 View
11 November 2009 5,381 0 View
i am doing Phd and cloning few genes from yeast i get partial sequence by standered methods than i did inverse pcr after autoligattion but fail than i did RT PCR by using SMART RACE after...
11 November 2009 1,043 4 View
hi friends i am cloning some genes form yeast i isolates total RNA which is of good quality now i am using RT PCR using SMART Race but according to manual and my own addition even i am not still...
11 November 2009 577 10 View
hi friends i am new memeber
11 November 2009 2,690 3 View
