12 December 2009 1 3K Report

i wish you happy,full of joy and a wonderful new year 2010 to all of scienctific community

Aslam buzdar

More Muhammad Aslam buzdar's questions See All
I need detail description of pMRP9-1 GFP?

Hi All, I am planning to use pMRP9-1 GFP plasmid to study virulence of Pseudomonas. Can any one send me link or detail description of this plasmid as i am unable to find it. or can any suggest me...

01 February 2018 243 1 View

plasmid curing

Dear fellows, i need help regrading plasmid curing of my yeast. i am working with klyveromyces sp. my research is cloning and expression of killer toxin protein by this strain. i wana confirm...

09 October 2010 1,171 3 View

hi friends suggestion about my killer toxin protein

hope you will be fine. as you know we had been discussing about my high molecular protein which was killer toxin. i got spectra of that protein i mean i send to company for mass spectraphotometer....

08 September 2010 7,129 1 View

hi

hope you will be fine. as you know we had been discussing about my high molecular protein which was killer toxin. i got spectra of that protein i mean i send to company for mass spectraphotometer....

08 September 2010 1,461 1 View

plasmid or gDNA

Dear Friends i am cloning some genes responsible for killer toxin proteins. first i also lated my protein and then did SDS PAGE and checked it mw that was about 68kd.then i get its dequence by...

07 August 2010 3,549 7 View

plasmid or gDNA

Dear Friends i am cloning some genes responsible for killer toxin proteins. first i also lated my protein and then did SDS PAGE and checked it mw that was about 68kd.then i get its dequence by...

07 August 2010 6,458 2 View

problem in proteind isolation

hi friends i am doing purifiaction of some yeast killer toxin proteins.i have isolated and purify them by anion exchange chromatography.i got one peak of my protein then i did sds page and get...

05 June 2010 2,821 1 View

hi guys please help me in protein quantification

i am new in the field of protein isolation and quantification. i have to do Bradford's protein assay i got G- 250 coomess brillient blue dye form sigma but i don't know how to make its solution...

05 June 2010 4,958 3 View

can any help me to find a papwe

Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian goats Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian...

04 May 2010 760 1 View

you help is needed

1"Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor"by K Al-Aidroos - 1978 2-"Molecular structure of the cell wall receptor for killer toxin...

03 April 2010 2,622 3 View

Similar questions and discussions
Is there any way to calculate whole genome similarity of organism without the whole genome sequencing ?

is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?

05 July 2024 4,070 3 View

Have pathomorphological studies of the lungs been performed in patients with desminopathy?

It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?

15 June 2024 1,355 7 View

How can I decide the variant I found segregating with a genetic condition is novel or known?

I found a variant segregating with a genetic condition, this variant is listed in ClinVar (with no clinical significance), gnomAD, etc. However, I could not find any publication reporting the...

10 June 2024 7,606 3 View

Describe the principles of Population Genetics and evolution as applied to livestock breeding programs?

Describe the principles of Population Genetics and evolution as applied to livestock breeding programs

08 June 2024 2,924 0 View

What is the suitable concentration of hygromycin in tomato sp. Micro-tom genetic transformation?

I used hygromycin and kanamycin for selecting positive buds in Micro-tom genetic transformation. Kanamycin performed very well and I could get positive plants soon, but hygromycin is a little bit...

29 May 2024 6,911 0 View

Are there any literature sources for proving that Geotrichum silvicola colony morphology can be slimy?

Hi, I've recently come across the colony of Geothrichum silvicola (based on sequencing result) isolated from soil with slimy morphology, unlike the colony morphology I've found on the journal...

13 May 2024 1,356 8 View

Will this vector plasmid works well?

Hello I am currently engaged in research focusing on plant genetic transformation. As part of this endeavor, I have designed a comprehensive in silico plasmid cloning approach. The aim is to...

07 May 2024 5,119 3 View

Why I am not getting accumulated GFP in vacuole for om45 WT GFP yeast strains?

I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...

04 May 2024 6,463 0 View

Who (first) proposed/used/coined the term ‘translation’ in biology/genetics?

Who (first) proposed/used/coined the term ‘translation’ in biology/genetics? What is the history behind the use of the word? Thank you!

01 May 2024 4,528 10 View

Can you help in Linkage Analysis Tool?

Can anyone assist with running the Linkage Analysis Tool 'Easylinkage' or any alternative tool for conducting linkage analysis and calculating LOD scores?

06 April 2024 7,273 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close