hi friends,
i am doing protein test by bradfrod method. i made coomesbrilliant blue reagent G-250 according to standard protocol.but i did not get same colour of that reagnet as it is stated in protocol( perhaps pink or light red/brown) my reagent is of blue colour. i don't know what is wrong with this i did twice. please and body is there to help me.
Adriana Liceth Alvarez De la Hoz
Hi! I've made it too. When i made it the colour was light brown. I used ethanol and phosphoric acid. Finally, you must filter it, if you observe lumps.
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