hi guys - FAQS.TIPS

Muhammad Aslam buzdar @Muhammad_Buzdar

12 December 2009 1 4K Report

i wish you happy,full of joy and a wonderful new year 2010 to all of scienctific community

Aslam buzdar

Muhammad Farooq Sabar

thank you for your wishes and same from here.

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I need detail description of pMRP9-1 GFP?

Hi All, I am planning to use pMRP9-1 GFP plasmid to study virulence of Pseudomonas. Can any one send me link or detail description of this plasmid as i am unable to find it. or can any suggest me...

01 February 2018 243 1 View

plasmid curing

Dear fellows, i need help regrading plasmid curing of my yeast. i am working with klyveromyces sp. my research is cloning and expression of killer toxin protein by this strain. i wana confirm...

09 October 2010 1,171 3 View

hi friends suggestion about my killer toxin protein

hope you will be fine. as you know we had been discussing about my high molecular protein which was killer toxin. i got spectra of that protein i mean i send to company for mass spectraphotometer....

08 September 2010 7,129 1 View

hi

hope you will be fine. as you know we had been discussing about my high molecular protein which was killer toxin. i got spectra of that protein i mean i send to company for mass spectraphotometer....

08 September 2010 1,461 1 View

plasmid or gDNA

Dear Friends i am cloning some genes responsible for killer toxin proteins. first i also lated my protein and then did SDS PAGE and checked it mw that was about 68kd.then i get its dequence by...

07 August 2010 3,549 7 View

plasmid or gDNA

Dear Friends i am cloning some genes responsible for killer toxin proteins. first i also lated my protein and then did SDS PAGE and checked it mw that was about 68kd.then i get its dequence by...

07 August 2010 6,458 2 View

problem in proteind isolation

hi friends i am doing purifiaction of some yeast killer toxin proteins.i have isolated and purify them by anion exchange chromatography.i got one peak of my protein then i did sds page and get...

05 June 2010 2,821 1 View

hi guys please help me in protein quantification

i am new in the field of protein isolation and quantification. i have to do Bradford's protein assay i got G- 250 coomess brillient blue dye form sigma but i don't know how to make its solution...

05 June 2010 4,958 3 View

can any help me to find a papwe

Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian goats Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian...

04 May 2010 760 1 View

you help is needed

1"Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor"by K Al-Aidroos - 1978 2-"Molecular structure of the cell wall receptor for killer toxin...

03 April 2010 2,622 3 View

Does anyone know where one can buy 8161 glass capillaries for making patch pipettes?

Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore

04 June 2024 5,688 2 View

Problem: cell aggregation with Cytofix/Cytoperm BD Biosciences?

The challenge encountered lies in cellular aggregation and clumping subsequent to incubation with Cytofix/Cytoperm BD Biosciences. Despite attempts to mitigate the issue through pipetting, the...

12 March 2024 3,926 0 View

Isolation of rat mesenchymal stem cells (Fischer F455 rats) - protocol and surface markers?

Hi, We are trying to isolate rat MSCs from Fischer F455 rats. So far we tried a femur flush protocol to flush the bone marrow into a petri dish. However, we are wondering if we should do...

12 July 2023 4,684 0 View

Call of Editorial boards at Algerian Journal of Biosciences?

Call of Edotrial boards in Algerian Journal of Biosciences About more informations please visit the journal website https://www.journal.acse.science/index.php/ajb/index If you are interested...

17 April 2023 3,999 0 View

Philosophically, are stars alive?

In the field of biosciences we often get in contact with the questions: Are viruses alive, a form of life? Is fire a form of life? Stars also burn fuel and stay stable until their fuel is burned...

16 April 2023 7,233 1 View

Where to purchase viruses other than ATCC?

I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...

12 January 2023 4,248 2 View

Is it true that the molecular weight of asyn protein is 25kd in westen-blot ?

The protein was extracted from mouse tissue(brain)，marker is Thermo 26619. The ladder was about in 25kd.And the antibody is BD Bioscience#asyn antibody. I'm not sure if it was α-synuclein.

08 December 2022 9,772 4 View

How do I sequence my isolated virus?

I've grown my influenza straind in MDCK cells and have isolated them. Now I have to sequence my virus, my lab uses Eton Biosciences. Do I send in primers for each gene? Do I extract RNA from the...

18 May 2022 2,592 0 View

My TCF/LEF Reporter - HEK293 Cell Line (Wnt Signaling Pathway) is struggling to grow upon thawing. Has anyone had similar issue with this cell line?

The reporter cell line has been cultured following the suppliers recommendation (see below): Thaw Medium 1 (BPS Bioscience #60187): MEM medium (Thermo Fisher, #11095098) supplemented with 10% FBS...

09 January 2022 3,186 0 View

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences?

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences and if so could you please tell me if there was any problems regarding communications with the...

05 October 2020 1,928 2 View