i am cloning a gene from yeast.frist i designed degenrate primer and get partial gene successfully.thandesigned primer according to know sequence and i tried inverse PCR to get complete gene but unfortunatly fail. than i tried Smart Race after isolating Total RNA but no result at all.my RNA and primers are right. i dont know what is problem with my gene. i cehcked every to me was okay but no result. please suggest me how to get my gene cloned?

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