I need detail description of pMRP9-1 GFP?

02 February 2018 1 238 Report

Hi All,

I am planning to use pMRP9-1 GFP plasmid to study virulence of Pseudomonas. Can any one send me link or detail description of this plasmid as i am unable to find it. or can any suggest me if this is right plasmid to use this kind of study?

Asst.Prof.Muthanna H. Hussain

Be careful Man..

Badges
Science topic

More Muhammad Aslam buzdar's questions See All

plasmid curing

Dear fellows, i need help regrading plasmid curing of my yeast. i am working with klyveromyces sp. my research is cloning and expression of killer toxin protein by this strain. i wana confirm...

09 October 2010 1,056 3 View

hi friends suggestion about my killer toxin protein

hope you will be fine. as you know we had been discussing about my high molecular protein which was killer toxin. i got spectra of that protein i mean i send to company for mass spectraphotometer....

08 September 2010 7,033 1 View

hi

hope you will be fine. as you know we had been discussing about my high molecular protein which was killer toxin. i got spectra of that protein i mean i send to company for mass spectraphotometer....

08 September 2010 1,372 1 View

plasmid or gDNA

Dear Friends i am cloning some genes responsible for killer toxin proteins. first i also lated my protein and then did SDS PAGE and checked it mw that was about 68kd.then i get its dequence by...

07 August 2010 3,452 7 View

plasmid or gDNA

Dear Friends i am cloning some genes responsible for killer toxin proteins. first i also lated my protein and then did SDS PAGE and checked it mw that was about 68kd.then i get its dequence by...

07 August 2010 6,361 2 View

problem in proteind isolation

hi friends i am doing purifiaction of some yeast killer toxin proteins.i have isolated and purify them by anion exchange chromatography.i got one peak of my protein then i did sds page and get...

05 June 2010 2,737 1 View

hi guys please help me in protein quantification

i am new in the field of protein isolation and quantification. i have to do Bradford's protein assay i got G- 250 coomess brillient blue dye form sigma but i don't know how to make its solution...

05 June 2010 4,851 3 View

can any help me to find a papwe

Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian goats Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian...

04 May 2010 666 1 View

you help is needed

1"Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor"by K Al-Aidroos - 1978 2-"Molecular structure of the cell wall receptor for killer toxin...

03 April 2010 2,530 3 View

please help me finding this paper

A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton (Gossypium hirsutum L.) by Wan C. Y. and Wilkins T. A.

03 April 2010 1,547 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View