Hi All,
I am planning to use pMRP9-1 GFP plasmid to study virulence of Pseudomonas. Can any one send me link or detail description of this plasmid as i am unable to find it. or can any suggest me if this is right plasmid to use this kind of study?
Be careful Man..
Dear fellows, i need help regrading plasmid curing of my yeast. i am working with klyveromyces sp. my research is cloning and expression of killer toxin protein by this strain. i wana confirm...
09 October 2010 1,171 3 View
hope you will be fine. as you know we had been discussing about my high molecular protein which was killer toxin. i got spectra of that protein i mean i send to company for mass spectraphotometer....
08 September 2010 7,129 1 View
08 September 2010 1,461 1 View
Dear Friends i am cloning some genes responsible for killer toxin proteins. first i also lated my protein and then did SDS PAGE and checked it mw that was about 68kd.then i get its dequence by...
07 August 2010 3,549 7 View
07 August 2010 6,458 2 View
hi friends i am doing purifiaction of some yeast killer toxin proteins.i have isolated and purify them by anion exchange chromatography.i got one peak of my protein then i did sds page and get...
05 June 2010 2,821 1 View
i am new in the field of protein isolation and quantification. i have to do Bradford's protein assay i got G- 250 coomess brillient blue dye form sigma but i don't know how to make its solution...
05 June 2010 4,958 3 View
Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian goats Comparison of cottonseed cake and dried blood as nitrogen sources for growing Sudan Nubian...
04 May 2010 760 1 View
1"Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor"by K Al-Aidroos - 1978 2-"Molecular structure of the cell wall receptor for killer toxin...
03 April 2010 2,622 3 View
A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton (Gossypium hirsutum L.) by Wan C. Y. and Wilkins T. A.
03 April 2010 1,642 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View