33 Questions 88 Answers 0 Followers
Questions related from Mohammad Sharif Sarker
Hello Mate, What procedure would be the best to get active spores even after 6 months? At commercial level when I get Trichoderma spores in bulk amount I need to store it but unfortunately its...
22 August 2020 8,392 5 View
Dear Colleagues I need to know Risk Group (RG) of Rhizoctonia, Colletotrichum, Sclerotium, Fusarium, Bipolaris and Phytophthora. Regards Mohammad Sharif Sarker
21 April 2019 3,916 12 View
Dear Colleagues I am interested to use secondary metabolites of trichoderma to enhance plant growth and to control plant pathogens. So How may I isolate cerinolactone from broth culture and...
01 January 2018 7,157 22 View
This fungi first time produce white hyphae on PDA but later it turns into orange color. I think its spores are orange color. Hyphae septate.
20 May 2017 5,956 15 View
During field performance evaluation of biofertilizers such as nitrogen fixer, zinc solubilizers which factors may be responsible for better performance. As I have noticed during poly house trial...
18 May 2017 3,582 37 View
I got this fungi while I was trying to isolate Pyricularia. It produce orange color colony on PDA, Fast growing fungi, septate hypahe, spores are single celled.
15 May 2017 6,176 8 View
As part of my fungal entomopathogens based bioinsecticide development I got several Isaria fungi. Now its time to do insect bioassay with those isolates. I have gotten several journals and...
09 May 2017 170 10 View
Without using V-8 juice which protocol may be good to promote sporangia production of Phytophthora? Regards
30 April 2017 8,522 24 View
How may I get colloidal chitin from shrimp shell? Any easy protocol available?
26 April 2017 7,897 2 View
Last time I used several technique to inoculate rice such as making wound on rice stem and inserting pathogen, pathogen mixing with soil and transplanting rice seedling etc. But only wound making...
13 April 2017 5,816 50 View
This fungal colony is white in color, reverse side is yellowish. Media all time cracked when subcultured on PDA or NA. Produce macro and micro conidia. Conidia produce from a sharp pointed...
29 March 2017 5,558 5 View
DOC2 media composition with Bactopeptone- 3g, CuCl2- 0.2g, Crystal violet- 2mg, Agar- 15 g, Water- 1000 ml. pH- 10 with Na2CO3 and HC Problem is after autoclaving media does not get solid state....
22 March 2017 8,560 2 View
To identify streptomyces scabies I need to do biochemical test. So any protocol available would be helpful. Regards Shuvrah
19 March 2017 2,548 10 View
For quality control of beauveria I need to try out spore germination percentage data too. Any method or any suggestion? Regards Shuvrah
14 March 2017 9,588 17 View
During Phytophthora culture I got fusarium contamination and its occurring every time. So which antibiotic or media or temperature I may use or maintain to get Fusarium free...
09 March 2017 1,333 15 View
During rice experiment set with trichoderma and zinc solubilzing bacteria I used seedling dipping method. Now I want to apply second doses of trichoderma and zinc solubilizing bacteria but which...
07 March 2017 9,177 14 View
I got this fungal isolate from termite insects when I was trying to isolate entomopathogens. Dear colleagues please let me know what is the identity of this fungi. Regards Shuvrah
14 December 2016 6,546 10 View
Please advice me about identity of this fungus produced spores on SDA media. Regards Shuvrah Rehman
12 December 2016 9,548 24 View
after isolating Phytophthora problme arose because of absence of suitable media. After several months not sporangia got on PDA. So which media should be used or which factors should taken into...
02 November 2016 1,143 9 View
one of my Alternaira sp. collection. front and reverse view of the pathogenic colony on PDA. single spore and multiple spore structure under microscope. what may the species of this...
18 October 2016 8,493 23 View
Well to do pathogenicity test of my new fungal isolate sclerotium I want to test with chickpea. So for getting better infection and perfect sclerotium symptom how may I start the pathogenicity test?
17 October 2016 8,567 6 View
Which protocol i may follow to preserve plant pathogens at -20 and at -80 degree Celsius specially Rhizoctonia, Alternaria, Bipolaris, Curvularia, Sclerotium, Colletotrichum and Fusarium? we are...
13 October 2016 8,576 14 View
this isolate got from rice. and whats identity? Regards Shuvrah
03 October 2016 6,581 7 View
Its the isolates which I am trying to identify. Today I got this germinating spores. I am suspecting it as Bipolaris. Am I right?
01 September 2016 8,596 14 View
I have four curvularia and four alternaria isolates. But I dont know the genus of those isolates. is there any website or researcher which/who deals which with these two pathogens? Or any group...
29 August 2016 739 2 View
To avoid insecticide entomopathogens are alternate way. I have been working with many samples like soil from different sources, plant parts, dead insects etc. But till now my target fungi...
22 August 2016 6,351 34 View
Which factors promote trichoderma spore production? Can I get few articles about it? I want to increase 108 spore/ml within shortest time of culture.so which salt or organic sources...
15 August 2016 1,738 4 View
From my collection of Curvularia fungi isoaltes I want to check pathogenicity. Which crop/age of seedlings/techniques of inoculation I may use to get best result? Thanks Shuvrah Rehman
04 August 2016 8,842 3 View
After completing PCR we stored all Tips, tissue papers, gloves etc in a storage box. Now its completely covered. So which method will be best to dispose it into the environment?
02 August 2016 9,358 3 View
I did pathogenicity test of Bipolaris in vitro with rice and wheat. In both cases Bipolaris produced typical symptoms. I used cotton swab method for pathogenicity test. But now i need to test in...
27 July 2016 5,871 4 View
for pathogenicity test of Bipolaris I need spore to spray on target crops. Unfortunately in PDB or in PDA this pathogen is not producing spore. But i got spore for the first time and after...
26 July 2016 6,029 0 View
Usually I need 6-7 days to get 106-107 spores/ml. But for industrial production its very long time. I want to produce maximum short duration. so I need suitable media with cheapest sources. Can...
25 July 2016 838 2 View
I used CTAB and other antibiotics to kill bacteria. another problem is no clear images of Beauveria sp. and Metarhizium sp. are available to justify my isolates.
06 April 2016 2,417 6 View
