I used CTAB and other antibiotics to kill bacteria. another problem is no clear images of Beauveria sp. and Metarhizium sp. are available to justify my isolates.
Hi Shuvrah,
I see you have already tried CTAB, attached is a paper which might help (second one).
You can also see the methods of the first paper. They used Sabouraud’s dextrose yeast agar to isolate. Infection of the insect was done using the soil-baiting technique. I am not sure, however, if the media is also available in your country.
The third one I got from ResearchGate (CTC medium), maybe you can also try contacting the authors if the problem persists.
Best of luck!
http://www.efflatounia.com/files/03-02.pdf
http://www.mycologia.org/content/104/4/974.short
Article CTC medium: A novel dodine-free selective medium for isolati...
Good day fellow,
I think you need to be specific on which of the entomopathogens you are working with whether its nematodes, viruses, parasitoids bacteria or fungi. If you are using fungi, i think common media isolation media like PDA or SDA can serve the purpose except you the medium you are using is selective; and should it be selective, you wouldnt need any antibiotics for bacterial inhibition.
Also, you should know that combination of these two antibiotics or using them at a concentration much higher than expected could culminate an impediment to your isolates.
Thank you
For isolation entomopathogenic fungi like Beauveria bassiana and Metarhizum anisopliae the best media is Potato dextrose agar (PDA) and potato dextrose broth, both are common microbiological growth media made from potato infusion, and dextrose, with Chlortetracycline, Chloramphenicol and tartaric acid.
Like Luis, we also use PDA in our lab for the maintenance of Beauveria and Metarhiium. Insect cadavers are placed on top of the agar and await for hyphal growth. For multiple growth, suspected entomopahtonegic fungi are re-streaked for purification and other colonies suspected to be saprophytic are ignored. Then you can use the purified colonies to re-infect fresh insects and re-isolate to confirm the identity.
Hello Dear
I think you have to use selective media.Selective media are used for the growth of only selected microorganisms. For example, if a microorganism is resistant to a certain antibiotic, such as ampicillin or tetracycline, then that antibiotic can be added to the medium in order to prevent other cells, which do not possess the resistance, from growingSelective growth media for eukaryotic cells commonly contain neomycin to select cells that have been successfully transfected with a plasmid carrying the neomycin resistance gene as a marker. Gancyclovir is an exception to the rule as it is used to specifically kill cells that carry its respective marker, the Herpes simplex virus thymidine kinase
Examples of selective media include:
Eosin methylene blue (EMB) contains dyes that are toxic for Gram positive bacteria. EMB is the selective and differential medium for coliforms
YM (yeast extract, malt extract agar) which has a low pH, deterring bacterial growth
MacConkey agar for Gram-negative bacteria
Hektoen enteric agar (HE) which is selective for Gram-negative bacteria
Mannitol salt agar (MSA) which is selective for Gram-positive bacteria and differential for mannitol
Terrific Broth (TB) is used with glycerol in cultivating recombinant strains of Escherichia coli.
Xylose lysine deoxycholate (XLD), which is selective for Gram-negative bacteria
Buffered charcoal yeast extract agar, which is selective for certain gram-negative bacteria, especially Legionella pneumophila
Baird–Parker agar for Gram-positiveStaphylococci
Hi @ Masoud Latifian
Thank you. I got one Beauveria for the first time on PDA. Thank you very much for your nice suggestion.
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