Well to do pathogenicity test of my new fungal isolate sclerotium I want to test with chickpea. So for getting better infection and perfect sclerotium symptom how may I start the pathogenicity test?
In common bean we used the "straw test" for greenhouse inocululation with Sclerotinia sclerotiorum. It's pretty simple - use the end of a short length (4-5 cm) of a drinking straw to cut a small agar plug from the leading edge of the mycelium on a PDA plate, then place the drinking straw over the cut stem of the plant to be inoculated. If you put the straw over the stem in such a way that the agar plug is in direct contact with the cut stem end you generally get very good infection. The straw keeps the plug moist until the fungus has the opportunity to infect, and stapling the other end of the straw shut will help with this.
Others have used agar plugs directly on leaf axils, but I think the straw test is superior as it maintains moisture in the plug.
I've attached a paper from the Ferreira lab that describes the procedure.
I hope this info is helpful for you. Good luck with your research!
http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-94-7-0885
Hello Jack Erron Haggard thanks for suggesting and attaching one valuable paper. Good luck.
Thanks Suresh K Malhotra but I need pictorial view of straw test. can you help me?
Here are some pictures from our straw test. The first is somewhat resistant, with infection stopping at the first node below the inoculation site. The second is more susceptible, with infection progressing beyond the first node and continuing to kill the entire plant. The last picture is of a PDA plate with plugs missing, sampled from the outside edge of the mycelium.
Jack your attached pictures are very much helpful. thank you
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