Its the isolates which I am trying to identify. Today I got this germinating spores. I am suspecting it as Bipolaris. Am I right?
What is the size of Conidiophores and Spores? Do you have culture picture?
Hello,
It's not easy to confirm it is Bipolaris, however the septa are not very obvious. It might be Curvularia.
Dear Shuvrah,
The colony's feature would not help so much to identify the fungus, also both Bipolaris and Curvularia have grey colonies. However, in your case you need to do more slides and and investigate them under the microscope, try to verify the septa of the conidia, if it 's pseudospetum (so you will recognize rings between the septa) that's may indicate it's Bipolaris. Please find the attached article concerning the genus Bipolaris. But still I am suspected with Curvularia which has conidia with true septa .
Good luck
Afra
Dear Afra Khiralla
I measured length of three spores. length is 3.42, 3.87 and 4.25 micro meter.
and this is germinating spores. if You look you will see germination starts from both end cells. I am sending few pictures of curvularia of my collection and spores are in germinating condition
In fact, the genera Bioplaris and Curvularia are more or less morphologically sisters. However, several species with a previously uncertain generic placement, such as Bipolaris subpapendorfii, B. neergaardii, B. nicotiniae, B. nodulosa, B. papendorfii, and B. portulaceae, are now placed in Curvularia based on phylogenetic analyses. So your morphological identification (either Bioplaris or Curvularia) is tentative identification, until you confirm it later using molecular characterization. I do not know if you have the possibility to do further investigations to re assess the fungus using molecular approach.
Good luck
Afra
Thank you Afra. Yes I have intention of molecular characterization but right now I cant do because few days back I did not get band after gel run. so right now I need to solve that problems of band forming.
I could not download your pdf file of "The Genus of Bipolaris". showed download failed. Any suggestion?
Best Regards
Shuvrah Rehman
You can confirm the species by use ITS primer,
this is a universal primer, then, you can use a specific primer
Thank you
Laith Al-Ani
Yes I will do sequencing but after few days. Now I am doing job with Beauveria.
Thank you
Dear Afra Khiralla
I downloaded the pdf file you sent me and its really very informative.
Thank you
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