after isolating Phytophthora problme arose because of absence of suitable media. After several months not sporangia got on PDA. So which media should be used or which factors should taken into consideration to maintain a good Phytophthora culture?
Dear Shuvrah Rehman
Pls. find the attached files
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC219352/
http://link.springer.com/article/10.1007/s12600-012-0218-4
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674107/
page 186 in: C. Booth . 1971. Methods in Microbiology, Academic Press, Pp.794. ISBN0080860303, 9780080860305
http://www.fs.fed.us/foresthealth/fhm/sp/sod/misc/culturing_species_phytophthora.pdf
Hoping this will be helpful
Regards
Prof. Houda Kawas
Very useful informations were shared among the researchers. Thanks Houda Kawas for sharing the documents
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Hi Shuvrah
V8 agar and broth are excellent growth media for species of Phytophthora and Pythium.
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