Greetings! I want to know about glass petri dish..
I used plastic petri dish at baking step of microarray, but it was distorted... so I want to use glass dish. But, is it Okay I reuse that dishes? in dishes, only dH2O injected about 3~4ml.
I think that after a cleaning followed by sterilization in autoclave of glass Petri dish there are not problems to re-use these.
Greetings. I cannot find about each component's role of CHURCH buffer at southern blotting(detail, pre-hybridization). I did DNA microarray but never have ever southern blot. In microarray,...
31 December 2018 993 0 View
Greetings. Here is sunday... In tris-glycine buffer, there are either include 1% SDS or not. I used former but many seller selling both Tris-glycine with SDS and without SDS. SDS is set charge...
31 December 2018 1,286 0 View
Greetings. Before question, I never did southern/nothern blot. (eastern too) During search buffer, I found Alkaline transfer buffer and knew it uses southern blot(alkaline method). This buffer...
31 December 2018 9,030 0 View
I saw 5X bingind buffer maded by; 100mM HEPES, pH 7.6 5mM EDTA 50mM ammonium sulfate 5mM DTT 1% Tween-20 150mM potassium chloride which reaction helps this buffer? I don't consider to this...
31 December 2018 4,996 0 View
Greetings. I heard about when make buffer include Tris, set pH using concentrated hydrochloric acid(37%). but SDS-PAGE, loading buffer(10X), Tris-glycine, what I heard set pH for 8.3, use only...
31 December 2018 4,043 3 View
Greetings. I extracted RNA at mouse liver and human blood, but degradation occurs. I think, at mouse liver, too hard to measuring 50mg liver(I am newbie for animal tissue preparation) so spend...
31 December 2018 4,731 0 View
Greetings. In image... left:ladder(start with 750bp) middle: PCR product with exonuclease III treated right(3rd lane): PCR product without exonuclease III treated there's a problem. PCR...
10 November 2017 9,923 12 View
I read a paper about PEG gel, . in this paper, supplymentary fig 10 is storage stability test. This particle maded by polyethylene glycol and store in 4 degree, but storage test is run at -20...
09 October 2017 2,894 0 View
Greetings. Experiments using microparticles are in progress. There are particles with multiple compositions and features, advisors want to compare the features of this particle finely, but I can...
08 September 2017 594 0 View
Guten morgen.(means 'Good mornong' in Deutsch) Recently I read a paper entitled "Circulating Tumor DNA Mutation Profiling by Targeted Next Generation Sequencing Provides Guidance for Personalized...
08 September 2017 5,138 1 View
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10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
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08 August 2024 6,134 12 View
I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?
08 August 2024 8,915 0 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
05 August 2024 8,056 1 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View