06 June 2017 0 7K Report

I want to find about molecular diagnostics, especially technique's evolution(history), and techniques that using nowadays.. but I cannot find something... Can you resommend article or websites written by I want to find things?

More Kyungsun Ha's questions See All
In CHURCH buffer, what components do role at southern blot?

Greetings. I cannot find about each component's role of CHURCH buffer at southern blotting(detail, pre-hybridization). I did DNA microarray but never have ever southern blot. In microarray,...

31 December 2018 993 0 View

In SDS-PAGE, how Tris-glycine buffer without SDS works?

Greetings. Here is sunday... In tris-glycine buffer, there are either include 1% SDS or not. I used former but many seller selling both Tris-glycine with SDS and without SDS. SDS is set charge...

31 December 2018 1,286 0 View

In alkaline transfer buffer, why put NaCl to alkaline transfer buffer?

Greetings. Before question, I never did southern/nothern blot. (eastern too) During search buffer, I found Alkaline transfer buffer and knew it uses southern blot(alkaline method). This buffer...

31 December 2018 9,030 0 View

What is 5X binding buffer?

I saw 5X bingind buffer maded by; 100mM HEPES, pH 7.6 5mM EDTA 50mM ammonium sulfate 5mM DTT 1% Tween-20 150mM potassium chloride which reaction helps this buffer? I don't consider to this...

31 December 2018 4,996 0 View

Why we don't have to use HCL/NaOH during make Trice-glycine buffer?

Greetings. I heard about when make buffer include Tris, set pH using concentrated hydrochloric acid(37%). but SDS-PAGE, loading buffer(10X), Tris-glycine, what I heard set pH for 8.3, use only...

31 December 2018 4,043 3 View

How can I measure tissues for RNA preparation without RNA dergadation?

Greetings. I extracted RNA at mouse liver and human blood, but degradation occurs. I think, at mouse liver, too hard to measuring 50mg liver(I am newbie for animal tissue preparation) so spend...

31 December 2018 4,731 0 View

What's the problem in gel electriphoresis?

Greetings. In image... left:ladder(start with 750bp) middle: PCR product with exonuclease III treated right(3rd lane): PCR product without exonuclease III treated there's a problem. PCR...

10 November 2017 9,923 12 View

Why stability test of PEG gel run at -20 degree?

I read a paper about PEG gel, . in this paper, supplymentary fig 10 is storage stability test. This particle maded by polyethylene glycol and store in 4 degree, but storage test is run at -20...

09 October 2017 2,894 0 View

Among the features of microparticles, what should be compared experimentally?

Greetings. Experiments using microparticles are in progress. There are particles with multiple compositions and features, advisors want to compare the features of this particle finely, but I can...

08 September 2017 594 0 View

What is Dunn's multiple comparison test?

Guten morgen.(means 'Good mornong' in Deutsch) Recently I read a paper entitled "Circulating Tumor DNA Mutation Profiling by Targeted Next Generation Sequencing Provides Guidance for Personalized...

08 September 2017 5,138 1 View

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