In CHURCH buffer, what components do role at southern blot?

Greetings. I cannot find about each component's role of CHURCH buffer at southern blotting(detail, pre-hybridization). I did DNA microarray but never have ever southern blot. In microarray,...

31 December 2018 885 0 View

In alkaline transfer buffer, why put NaCl to alkaline transfer buffer?

Greetings. Before question, I never did southern/nothern blot. (eastern too) During search buffer, I found Alkaline transfer buffer and knew it uses southern blot(alkaline method). This buffer...

31 December 2018 8,961 0 View

In SDS-PAGE, how Tris-glycine buffer without SDS works?

Greetings. Here is sunday... In tris-glycine buffer, there are either include 1% SDS or not. I used former but many seller selling both Tris-glycine with SDS and without SDS. SDS is set charge...

31 December 2018 1,224 0 View

Why we don't have to use HCL/NaOH during make Trice-glycine buffer?

Greetings. I heard about when make buffer include Tris, set pH using concentrated hydrochloric acid(37%). but SDS-PAGE, loading buffer(10X), Tris-glycine, what I heard set pH for 8.3, use only...

31 December 2018 3,971 3 View

What is 5X binding buffer?

I saw 5X bingind buffer maded by; 100mM HEPES, pH 7.6 5mM EDTA 50mM ammonium sulfate 5mM DTT 1% Tween-20 150mM potassium chloride which reaction helps this buffer? I don't consider to this...

31 December 2018 4,938 0 View

How can I measure tissues for RNA preparation without RNA dergadation?

Greetings. I extracted RNA at mouse liver and human blood, but degradation occurs. I think, at mouse liver, too hard to measuring 50mg liver(I am newbie for animal tissue preparation) so spend...

31 December 2018 4,671 0 View

What's the problem in gel electriphoresis?

Greetings. In image... left:ladder(start with 750bp) middle: PCR product with exonuclease III treated right(3rd lane): PCR product without exonuclease III treated there's a problem. PCR...

10 November 2017 9,858 12 View

Why stability test of PEG gel run at -20 degree?

I read a paper about PEG gel, . in this paper, supplymentary fig 10 is storage stability test. This particle maded by polyethylene glycol and store in 4 degree, but storage test is run at -20...

09 October 2017 2,833 0 View

Among the features of microparticles, what should be compared experimentally?

Greetings. Experiments using microparticles are in progress. There are particles with multiple compositions and features, advisors want to compare the features of this particle finely, but I can...

08 September 2017 535 0 View

What is Dunn's multiple comparison test?

Guten morgen.(means 'Good mornong' in Deutsch) Recently I read a paper entitled "Circulating Tumor DNA Mutation Profiling by Targeted Next Generation Sequencing Provides Guidance for Personalized...

08 September 2017 5,063 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View