Why we have to preheat hybridization buffer?

More Kyungsun Ha's questions See All

In CHURCH buffer, what components do role at southern blot?

Greetings. I cannot find about each component's role of CHURCH buffer at southern blotting(detail, pre-hybridization). I did DNA microarray but never have ever southern blot. In microarray,...

31 December 2018 993 0 View

In SDS-PAGE, how Tris-glycine buffer without SDS works?

Greetings. Here is sunday... In tris-glycine buffer, there are either include 1% SDS or not. I used former but many seller selling both Tris-glycine with SDS and without SDS. SDS is set charge...

31 December 2018 1,286 0 View

In alkaline transfer buffer, why put NaCl to alkaline transfer buffer?

Greetings. Before question, I never did southern/nothern blot. (eastern too) During search buffer, I found Alkaline transfer buffer and knew it uses southern blot(alkaline method). This buffer...

31 December 2018 9,030 0 View

What is 5X binding buffer?

I saw 5X bingind buffer maded by; 100mM HEPES, pH 7.6 5mM EDTA 50mM ammonium sulfate 5mM DTT 1% Tween-20 150mM potassium chloride which reaction helps this buffer? I don't consider to this...

31 December 2018 4,996 0 View

Why we don't have to use HCL/NaOH during make Trice-glycine buffer?

Greetings. I heard about when make buffer include Tris, set pH using concentrated hydrochloric acid(37%). but SDS-PAGE, loading buffer(10X), Tris-glycine, what I heard set pH for 8.3, use only...

31 December 2018 4,043 3 View

How can I measure tissues for RNA preparation without RNA dergadation?

Greetings. I extracted RNA at mouse liver and human blood, but degradation occurs. I think, at mouse liver, too hard to measuring 50mg liver(I am newbie for animal tissue preparation) so spend...

31 December 2018 4,731 0 View

What's the problem in gel electriphoresis?

Greetings. In image... left:ladder(start with 750bp) middle: PCR product with exonuclease III treated right(3rd lane): PCR product without exonuclease III treated there's a problem. PCR...

10 November 2017 9,923 12 View

Why stability test of PEG gel run at -20 degree?

I read a paper about PEG gel, . in this paper, supplymentary fig 10 is storage stability test. This particle maded by polyethylene glycol and store in 4 degree, but storage test is run at -20...

09 October 2017 2,894 0 View

Among the features of microparticles, what should be compared experimentally?

Greetings. Experiments using microparticles are in progress. There are particles with multiple compositions and features, advisors want to compare the features of this particle finely, but I can...

08 September 2017 594 0 View

What is Dunn's multiple comparison test?

Guten morgen.(means 'Good mornong' in Deutsch) Recently I read a paper entitled "Circulating Tumor DNA Mutation Profiling by Targeted Next Generation Sequencing Provides Guidance for Personalized...

08 September 2017 5,138 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View