Hi,

Yes, the Tris-HCl buffer mostly helps buffer the pH of your solution. It's quite relevant, especially with RNA, to increase it's stability and allow some storage time.

The pH and concentration of your buffer is very dependant on the rest of your protocol, so it should be mentionned in any protocol you chose. Be sure to follow those specifications, as a different pH could alter the efficiency of some column-based extractions, and a higher salt concentration could lower efficiency of some enzyme reactions (like your RT reaction, DNase, PCR, etc.)

You can make a concentrated stock solution of your Tris-HCl buffer and dilute it before use, to save some time.

Hope this helps, good luck!

Dear Kiriakos Athanasiadis ,

The Tris-HCl is a common biological buffer used to maintain a stable pH in various biochemical and molecular biology applications, including RNA isolation. Its pH buffering capacity helps maintain a stable environment, which is crucial for preserving the integrity of RNA during the isolation process.

To determine the appropriate concentration of Tris-HCl for RNA isolation, it's essential to know the specific RNA isolation protocol you will be using. Different protocols may require different concentrations of Tris-HCl depending on the method and the other components in the buffer.

Hope this will answer to your question,

Best regards

Tris HCl buffer is commonly used in RNA isolation protocols due to its ability to stabilize the pH level, which helps maintain the integrity and stability of RNA during the isolation process.

Tris HCl is a popular choice as it resists pH changes when exposed to acidic or basic conditions, providing a consistent environment for RNA extraction.

The typical concentration of Tris HCl used in RNA isolation is around 10-100 mM, depending on the specific protocol or application.