Bio-Rad iQ-Check Gluten PCR Kit

Commercial RT-PCR/qPCR Kits for Gluten Source Detection

✅ 1. R-Biopharm (Germany) – SureFood® ALLERGEN kits

• Kit Name: SureFood® ALLERGEN Gluten (Art. No. S3606)
• Detection: Qualitative and quantitative detection of wheat, barley, and rye DNA.
• Technology: Real-time PCR (qPCR)
• Validated For: Raw and processed foods (e.g., baked goods, flours, spices)
• Key Features: High specificity and sensitivity ISO 17025-compliant protocols available
• Website

✅ 2. BIOTECON Diagnostics – foodproof® Grain Kits

• Kit Name: foodproof® Gluten Detection Kits
• Detection: Can differentiate DNA from wheat, barley, rye, oats, and others
• Technology: qPCR, multiplex compatible
• Applications: Food, feed, raw material screening, contamination analysis
• Kit Variants: Species-specific kits also available

✅ 3. Primerdesign™ (UK, now part of Novacyt) – Genesig® Kits

• Kit Name: Genesig® Gluten (GMO) Detection Kit
• Detection: Designed to detect gluten-specific genes (used mostly in food safety and allergen testing)
• Technology: RT-qPCR
• Customization: Species-specific assays may be available on request

✅ 4. Eurofins GeneScan / GeneScan Technologies

• While not selling kits directly, they provide: Validated detection services using qPCR for gluten sources Custom assay development for in-house use if needed

🔍 Scientific Basis

• These kits generally detect conserved genomic sequences in prolamin genes: Gliadin – wheat Secalin – rye Hordein – barley

Using RT-qPCR allows for both:

• Qualitative identification (presence/absence of a gluten source)
• Quantitative estimation (amount of contaminant DNA)