How can i break efficiently Chlamydomonas reinhardtii cells for metabolites and enzyme activity determination?

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Need to obtain concentration of DNA/RNA?

I need to use a photometer, so i have Cary Agilent and i would like to know what kind of cuvettes and its dimensions and volume i have to order. It's about RNA and DNA samples. Which are you...

11 June 2024 712 1 View

I have some questions for calculation working with GC MS, help?

So, i m working during last month with GC-MS. When i'm making the extraction of samples e.g. PAH samples, i'm pipetting significant concentration of two internal controls into the solution. When i...

31 March 2024 6,551 1 View

How can i transform copies DNA to mg/kg?

I am working with an allergen and i am working using PCR, the result that offers the kit is copies DNA, although i need to give a result in mg/kg. Is any possible way? Thank you in advance,...

24 March 2024 5,657 3 View

Question about calculation for Standards GC-MS?

I have 4 substances for PAH, each one is 100ppm or 1000ppm, i would like to make a mix(4-PAH) of them of final volume 1mL and final concentration 100μg/kg. How can i proceed? Thank you in advance,

11 March 2024 5,660 2 View

Determination of Dioxins/Furans via GC-MS?

Hallo! I m working on GC-MS instrument these days and i am installing some methods. I would like to ask if you know any research paper or directly a method for Dioxins/Furans via GC-MS?

06 March 2024 7,843 2 View

I am using BIO0-RAD Tubes and Caps for my PCR Reactions, so when PCR starts the tubes are opened (95Celsius). What i have to do?

I am using PCR Instrument called SLAN from HONGSHI. Any clue?

23 January 2024 9,713 7 View

May i use knock out gene method to obtain confirmation for gene expression?

I would like to confirm PCR Results, so i thought that "knock out" of gene of interest will work. Who support this? If someone has different aspect would you please share?

17 January 2024 1,445 2 View

May i use Northern blot to ensure my RT-PCR results?

Is it highly recommended to use northern blot to ensure my RT-PCR results instead of LC-MS for example? Thank you in advance, Kiriakos

03 December 2023 1,795 2 View

What i have to pay attention in gene expression interpretation?

I m gonna make an excel for results interpretation for gene expression on food matrixes for quantification. How can i proceed? Thanks you in advance, Kiriakos

03 December 2023 5,814 0 View

How to convert Citric acid to Calcium citrate?

I have 10mg/kg obtained by using HPLC, How to convert to Calcium citrate? Thank you,

12 October 2023 4,331 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View

How to purchase metabolites of pesticide standards ?

Penconazole 1. CGA 132465 2. CGA 190503 3. CGA 127841 Fluopyram 1. AE C656948-benzamide 2. AE C656948-pyridyl-acetic acid 3. AE C656948-carboxylic acid I have searched on sigma aldrich as well...

09 July 2024 3,414 2 View

Does plant based medicine have less side effects?

I was reading papers on plant secondary metabolites and their applications and many of the papers/books I read, have the point that plant-based medicines which are the result of application of...

08 July 2024 9,935 10 View

How is the preparation to obtain secondary metabolic extracts?

Hi everyone! I am working with bacteria and fungal collected from marine sediments. I would to know the methodology since the preparation of biomass until the extraction and stored of secondary...

03 July 2024 6,177 3 View

What statistical test to do for dose response graphs?

Hello, I am currently conducting research on ovarian tumour cells and the use of chemotherapies with dietary metabolites and their combinations to see if there is a difference in cell viability. I...

01 July 2024 6,100 2 View