What is the best way or technique to measure the concentration of 20 nm gold nanoparticles per gram of brain areas tissues ?
Dear Heba
Without prejudice I think working on low concentrations in animal tissues require adequate equipment. I am of the same opinion as Wang Xin using ICP - MS
Best regards
Djamel
Dear Heba
Without prejudice I think working on low concentrations in animal tissues require adequate equipment. I am of the same opinion as Wang Xin using ICP - MS
Best regards
Djamel
I think it is better to use ICP-MS. If not available, you can also use atomic absorption spectrophotometry
Kindly check this paper
Kehoe DF, Sullivan DM, Smith RL. Determination of gold in animal tissue bygraphite furnace atomic absorption spectrophotometry. J Assoc Anal Chem 1988;71(6):1153–5.
In my opinion, you can digest an accurate weight of the brain sample properly in a proper system like perchloric-HCl. Re dissolve the solid residue in minimum amount of deionized water and analyse you sample by ICP-MS.
Kind regards
I agree ICP would be the best method but sample preparation is not as easy as it seems.
A different approach (if you don`t have an access to ICP-MS) could be taking the tissue and putting it to H2SO4 to do the electrochemical experiment. The curve for reduction-oxidation of gold is unmistakable and from the hight of the peak you can find the exact amount of gold in your sample.
I agree ICP-MS or ICP-OES (depending on the concentration) and you can try to digest you samples by using perchloric acid at high temperatures (approx 180ºC). Using this you can evaluate the Au ions concentration and then you can estimate the amount of particles you had in the original tissue.
I think you can use CT or sacrifing them and count their tissue via ICP after sample preparation
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