I prepared TPP chitosan and loaded it with thymoquinone drug, the zeta potential was about 30 mv , when I made coat with tween 80 to this combination, zeta potential dropped to 4
Isa there any explanation ?
I need a certain method to cause irreversible DNA denaturation in double stranded FIsh DNA
01 February 2019 6,717 3 View
I'm preparing niosome encapsulating a certain natural extract how can I caclulate the encapsulated dose which is equivalent to the free dose of that substance?
11 December 2018 7,655 3 View
I want to treat rats with a certain drug loaded on a nanocarrier with a certain dose... The problem the i have.. That when converting my formulation to powder through air drying.. The yield is...
01 February 2018 7,203 3 View
I want a simple protocol ( non-costly) for the separation of total proteins from breast tissues without altering their conformations
01 February 2018 5,784 1 View
Would someone suggest a simple protocol for total proteins extraction from human tissues that don't alter protein conformation ?
01 February 2018 3,006 2 View
What is the best solvent for IP injection in rats for TQ for one week treatment ?
01 February 2018 1,059 1 View
what is the best solvent for chitosan nanoparticles for IP injection in rats for one week treatment ?
01 February 2018 7,361 2 View
Would someone tell me a successful simple protocol for DNA isolation from animal tissues , that gives reasonable yield and which is not costly and does not alter DNA structure ?
01 February 2018 8,545 7 View
What is the simple organism or cell type from which I can isolate its membrane and what is the best model to study the interaction of nanoparticles with cell membrane ?
31 December 2017 4,319 3 View
I will make an in vivo experiment in which I want to compare between the effect of a free drug and the same drug loaded on chitosan nanoparticles.. how can I calculate the dose of nanoparticles...
31 December 2017 4,358 2 View
[Synthesizing Cu nanoparticles without using chemical reductants]
01 March 2021 8,359 1 View
Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...
01 March 2021 8,220 2 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View
Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax
28 February 2021 6,685 2 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
28 February 2021 274 2 View
I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...
27 February 2021 1,829 3 View
It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?
26 February 2021 1,760 8 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...
25 February 2021 7,994 3 View