I want to make ICP for nanometal detection in biological tissues, the first step is the tissue digestion, is there is any easy protocol or method to follow it ?
I need a certain method to cause irreversible DNA denaturation in double stranded FIsh DNA
01 February 2019 6,800 3 View
I'm preparing niosome encapsulating a certain natural extract how can I caclulate the encapsulated dose which is equivalent to the free dose of that substance?
11 December 2018 7,710 3 View
I want to treat rats with a certain drug loaded on a nanocarrier with a certain dose... The problem the i have.. That when converting my formulation to powder through air drying.. The yield is...
01 February 2018 7,258 3 View
I want a simple protocol ( non-costly) for the separation of total proteins from breast tissues without altering their conformations
01 February 2018 5,835 1 View
Would someone suggest a simple protocol for total proteins extraction from human tissues that don't alter protein conformation ?
01 February 2018 3,058 2 View
What is the best solvent for IP injection in rats for TQ for one week treatment ?
01 February 2018 1,114 1 View
what is the best solvent for chitosan nanoparticles for IP injection in rats for one week treatment ?
01 February 2018 7,408 2 View
Would someone tell me a successful simple protocol for DNA isolation from animal tissues , that gives reasonable yield and which is not costly and does not alter DNA structure ?
01 February 2018 8,606 7 View
I will make an in vivo experiment in which I want to compare between the effect of a free drug and the same drug loaded on chitosan nanoparticles.. how can I calculate the dose of nanoparticles...
31 December 2017 4,413 2 View
What is the simple organism or cell type from which I can isolate its membrane and what is the best model to study the interaction of nanoparticles with cell membrane ?
31 December 2017 4,375 3 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this
05 August 2024 7,902 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View