How to evaluate inductance of a solenoid in accordance with the air gap?

Hello All, I am currently modeling a solenoid on Matlab. I have evaluated the inductance of the solenoid based on its geometrical configuration using the formula, L = mu0*n^2*V where, mu0 =...

16 February 2021 5,613 5 View

How to define acceptable log10 range for real time pcr quantitative assay?

Case: I have performed inter laboratory comparison for 2 samples and obtained our lab and referral lab value in IU/ml. As per NABL guidelines (India) the acceptable range between the two values...

25 November 2020 4,112 1 View

How to run the MD simulation for a protein-ligand system in NAMD?

Hi I am trying to run the MD simulation for protein-ligand system in NAMD. But I am having troubles running it. It says the ligand is not parametrised when generating psf file. I understood the...

28 August 2019 9,774 2 View

Validation of docking result?

hello. i have finished running the ensemble docking with ligand library. what are the next step of computational methods or other methods available to prove the top scored molecules likely to be...

08 March 2019 3,175 7 View

With a 405 nm CW laser, What are the suitable fluence values to reduce graphene oxide free standing films?

I am trying to reduce the graphene oxide free standing films(50 micron thick) with a 405 nm CW laser. The laser has an average power of 534 mW. I am looking for some suggestions for what fluence...

11 April 2018 8,184 1 View

Which is the best inhouse protocol for midi and maxi prep plasmid DNA isolation?

Looking for midi and maxi plasmid isolation protocols from E coli (high copy number plasmid) cultures above 100ml (LB) Protocols tried so far: Sambrook's midi and maxi prep Alkaline lysis...

04 April 2018 1,377 3 View

What can improve copy number detection sensitivity of real time pcr FAM-BHQ1 probe based?

I am working with development of viral diagnostic kits using Real Time PCR (Probe based) detection. As per standard procedures we do use plasmid carrying viral gene as control and to determine the...

29 November 2017 1,758 21 View

How to calculate acoustic emission signals to stress generation while machining

I want to calculate stress generation with respect to load acting on the member while machining with abrasive waterjet machine by using AE signals. Can anyone help?Thanks

27 February 2017 5,678 3 View

Efficient and fast RBC lysis protocol.? (NOT Commercial kit method)?

Please share protocols for faster and efficient lysis of RBC. Probable requirements: lysis buffer to sample (volume) ratio 1:4/1:2

19 January 2017 2,158 9 View

Name few best fermentation medium compositions to attain high cell density in Bacillus subtilis strains.?

I am working with Bacillus subtilis RIk1285 (derivative of 168 W.T from TaKaRa), the maximum O.D I get when cultured in L.B broth (Flask work) is 4 O.D and in 7.5 Litre bench top fermentor...

09 June 2016 768 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

Can any scholar assist me with an r code for calculating average bias for each parameter of a continuous distribution via simulation?

I want to perform a simulation study to examine the consistency property of parameters of a new distribution through the quantile function. The simulation study will contain many replicates (say...

10 February 2021 180 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Does diseases like fungal,bacterial etc of rice plant is different from nutrient deficiency diseases?how deep learning is used except classification?

can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...

07 February 2021 470 2 View

Should we analyze or relinquish the clinically confirmed data of COVID-19?

Hello everybody As you know, due to the high cost and time-consuming of laboratory assays, like PCR, for the suspected cases of COVID-19, doing this type of investigation is not possible for all...

06 February 2021 6,122 3 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View