Bring a solution of SYBR Green–stained

DNA up to 100 mM NaCl and add 2½ volumes of absolute or 95% ethanol. After incubating for about 20 minutes on ice, centrifuge mixture for at least 10 minutes in a microfuge at 4°C. Remove the ethanol and wash the pellet once with 70% ethanol. Evaporate the ethanol and resuspend double-stranded DNA in TE.

Hello Dr. Anish Anna

Thank you for the protocol :) will try and let u know the results.

Hello Ali

No I want to purify just ETBR or SYBR stained DNA sample. Like mixing eluted genomic DNA with ETBR and do some process and later get rid of it .

Similar to what Dr. Anish said.

May be you can denature the DNA at 95 degrees for 10 minutes then add either 95% ethanol or isopropanol to precipitate DNA. Centrifuge, remove supernatant and dilute you DNA. You might end up with pure. 

I think PCR amplicon purification or gel extraction will also remove the EtBr or SYBR dye.

Hello Vikram bhai

I want to get rid of ETBR/SYBR not from agarose gel but from isolated DNA which is stained with ETBR for some other process (apart from agarose gel) and once the process is done I wish to get rid of ETBR/SYBR.

Any ways heat-wash step you have mentioned I shall give a try.

THank You

Hi Goutham,

My primary answer is heat-wash method. Second method depends on the mode of sample you have.

No I have some other ideas for etbr/sybr staining not the CsCl thing Ali.

Ok I will make a note of shared procedures