Standard phenol:chloroform extraction together with alcohol precipitation yield good-quality DNA. http://cshprotocols.cshlp.org/content/2006/1/pdb.prot4455.long

It is a simple, robust and scalable method.

There are several possible issues that should be concerned:

1. Extraction from small volumes is tend to result in high loss of DNA. Therefore, it is recommended to work with at least 100 μL volumes.

2. In the case of low DNA concentrations using of glycogene, linear polyacrylamide and some other agents can help precipitation and increase the DNA yield. It also should be noted that some of this compounds can hinder several protocols.

3. In many protocols isoamyl alcohol is required for better protein extraction. However, it can be avoided.

Hello Igor thanks for ur inputs

How about a protocol like this:

PCR product volume 200 ul

Spun down at 10,000 RPM for 10 minutes at 4 C

supernatant to fresh tube

100% Iso propanol wash

Discard the supernatant

2 70% ice cold ethanol washes

Air dry and resuspened in TE/Water?

Are there any technical ambiguities in the end product obtained using above procedure?

Actually, alcoholic precipitation can be performed at room temperature, when concentration of DNA is high. Isopropanol wash also can be skipped, 2x ethanol wash is enough to remove traces of salts and isopropanol in sediment. However, direct precipitation of PCR probes will result in precipitation not only DNA, but also proteins, including Taq. In some cases (endonuclease digestion for cloning) it is believed that Taq can hinder further processing of DNA. Therefore, phenol:chloroform extraction or other method are needed to remove proteins before precipitation.

Which method of DNA purification is better for sequencing Column or agarose gel

Try Exo-Sap method:

https://www.thermofisher.com/order/catalog/product/78205.10.ML#/78205.10.ML

Can exosap remove primer dimer contamination in PCR product?