How to evaluate inductance of a solenoid in accordance with the air gap?

Hello All, I am currently modeling a solenoid on Matlab. I have evaluated the inductance of the solenoid based on its geometrical configuration using the formula, L = mu0*n^2*V where, mu0 =...

16 February 2021 5,613 5 View

How to define acceptable log10 range for real time pcr quantitative assay?

Case: I have performed inter laboratory comparison for 2 samples and obtained our lab and referral lab value in IU/ml. As per NABL guidelines (India) the acceptable range between the two values...

25 November 2020 4,112 1 View

How to run the MD simulation for a protein-ligand system in NAMD?

Hi I am trying to run the MD simulation for protein-ligand system in NAMD. But I am having troubles running it. It says the ligand is not parametrised when generating psf file. I understood the...

28 August 2019 9,774 2 View

Validation of docking result?

hello. i have finished running the ensemble docking with ligand library. what are the next step of computational methods or other methods available to prove the top scored molecules likely to be...

08 March 2019 3,175 7 View

With a 405 nm CW laser, What are the suitable fluence values to reduce graphene oxide free standing films?

I am trying to reduce the graphene oxide free standing films(50 micron thick) with a 405 nm CW laser. The laser has an average power of 534 mW. I am looking for some suggestions for what fluence...

11 April 2018 8,184 1 View

Which is the best inhouse protocol for midi and maxi prep plasmid DNA isolation?

Looking for midi and maxi plasmid isolation protocols from E coli (high copy number plasmid) cultures above 100ml (LB) Protocols tried so far: Sambrook's midi and maxi prep Alkaline lysis...

04 April 2018 1,377 3 View

What can improve copy number detection sensitivity of real time pcr FAM-BHQ1 probe based?

I am working with development of viral diagnostic kits using Real Time PCR (Probe based) detection. As per standard procedures we do use plasmid carrying viral gene as control and to determine the...

29 November 2017 1,758 21 View

Would like to know where I can buy the standards for HBV,HCV, HIV to perform Real-Time PCR based diagnosis?

What are the companies or government bodies in India who can provide international standards for Hepatitis B, C, and HIV which can be used for quantitiative measurement using Real -Time PCR .

23 June 2017 2,855 4 View

How to calculate acoustic emission signals to stress generation while machining

I want to calculate stress generation with respect to load acting on the member while machining with abrasive waterjet machine by using AE signals. Can anyone help?Thanks

27 February 2017 5,678 3 View

Efficient and fast RBC lysis protocol.? (NOT Commercial kit method)?

Please share protocols for faster and efficient lysis of RBC. Probable requirements: lysis buffer to sample (volume) ratio 1:4/1:2

19 January 2017 2,158 9 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View