I here attach the reference for bacillus transformation using paris method.
It says MG1 and MG2 salts need aminoacid growth factor to 50 ug/ml and 10 ug/ml.
I do know yeast extract is a source of amino acids, but i here get confused with the word amino acid growth factor.
Any one who worked with paris method please clarify this.
thank you.
The amino acid requirement depends on the strain. If you are working with strain 168, you need to add tryptophan. If you are working with a strain like PY79 or W168, no amino acids are required. Other strains could have other requirements. You need to be certain you understand the strain's genotype.
By the way, the Paris method works for Bacillus subtilis 168 and its derivatives. It also works, with lower efficiency, for certain strains of B. licheniformis and B. amyloliquefaciens subsp. plantarum. Most natural isolates of Bacillus sp. cannot be made competent this way.
Dear Daniel
Thank You, I am working with Bacillus subtilis 1A751 strain from BGSC which is a derivative of 168. By the time i posted this question i did a try transformation with giving only casamino acids and no growth factor. Just a while ago i did get good transformants. Thank You for your suggestion
I will have to try for licheniformis the same.
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