Abhilek Kumar Nautiyal

17 Questions 36 Answers 0 Followers

Questions related from Abhilek Kumar Nautiyal

How to confirm that NADPH is working fine or not oxidised?

In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?

30 April 2023 2,331 0 View

Any protocol for Glycerol and Sucrose estimation?

Can anybody please share the Glycerol and Sucrose estimation protocol? I want to check the Glycerol and Sucrose concentration in the yeast culture. I really appreciate any help you can provide.

27 December 2022 7,926 4 View

Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ?

Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ? There are many solvents, but why only phenol-chloroform is used...

05 December 2022 4,141 2 View

Can anyone please provide me with PAGE gel recipe for RNA ?

Hello everyone I am looking for a PAGE gel protocol/recipe for RNA.

07 April 2022 2,311 2 View

How to measure or calculate milliarbitrary units (mAU) of RNAse ?

Hello Researchers I need to measure or calculate milliarbitrary units (mAU) of RNAse? A total of 3.8 mAU of RNAse (Life Technologies) per μg of RNA was added to the formulations Can anybody please...

24 March 2022 466 1 View

What is the Zeta potential of mRNA?

Can anybody brief me on the zeta potential of mRNA?

27 December 2021 8,827 4 View

Extra band in Gel elution?

I have amplified a gene of interest by PCR. On successive amplification, I am getting a single band of the correct size. But, when I cut the particular band from the gel and perform elution with...

17 November 2021 2,847 3 View

Can anyone explain me how to make 5% DMSO solution?

I want to make 5% DMSO solution for my substrate such as Fatty acids and naphthalene to dissolve.

05 April 2019 2,466 6 View

Protocol for extraction and derivitization of fatty acid for GC analyis?

I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.

27 January 2019 7,786 11 View

How to fuse two independent domain?

I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?

11 October 2018 1,779 4 View

Facing problem in Cloning?

facing problem in cloning.No colony in transformants plate.

20 August 2018 6,291 9 View

Add ribosome binding site in primers?

I am adding ribosome binding site (GGAG) in primer to direct protein synthesis.Is this affect the protein synthesis.I am using pET28a vector?

27 February 2018 1,770 0 View

Protocol for cell lysis BL21 (DE3) by sonication?

I want to break cell BL21 (DE3) for protein estimation by sonication method.What are the differnt conditions,buffers used for this methos?

02 June 2017 2,242 1 View

How to identify BL21 vs BL21(DE3) strain?

23 May 2017 4,295 4 View

Do we express our recombinant protein into TOP10 instead of BL21(DE)?

Do we express our recombinant protein into TOP10 instead of BL21(DE)? Is this affects its protein expression?

19 April 2017 6,620 2 View

How to isolate gram +ve bacterial genomic dna with high conc?

I want to isolate +ve bacterial genomic dna with high conc.I am using SIGMA kit for isolation?

01 January 1970 3,938 5 View

I m trying to break bacillus megaterium cell with high pressure homogeniser..bt notng happen to my cell...i continued upto 2.30 hr?

Buffer composition 50mM tris hcl Nacl 150mM Mgcl2 5mM 10%glycerol

01 January 1970 2,473 3 View