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Questions related from Abhilek Kumar Nautiyal
The Superdex S75 10/300 column is exhibiting abnormally high backpressure, with the system pressure rising to ~160 psi while maintaining a very low flow rate of only 0.040 mL/min. Despite multiple...
last month 4,345 2 View
In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?
30 April 2023 2,438 0 View
Can anybody please share the Glycerol and Sucrose estimation protocol? I want to check the Glycerol and Sucrose concentration in the yeast culture. I really appreciate any help you can provide.
27 December 2022 8,036 4 View
Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ? There are many solvents, but why only phenol-chloroform is used...
05 December 2022 4,224 2 View
Hello everyone I am looking for a PAGE gel protocol/recipe for RNA.
07 April 2022 2,389 2 View
Hello Researchers I need to measure or calculate milliarbitrary units (mAU) of RNAse? A total of 3.8 mAU of RNAse (Life Technologies) per μg of RNA was added to the formulations Can anybody please...
24 March 2022 540 1 View
Can anybody brief me on the zeta potential of mRNA?
27 December 2021 8,914 4 View
I have amplified a gene of interest by PCR. On successive amplification, I am getting a single band of the correct size. But, when I cut the particular band from the gel and perform elution with...
17 November 2021 2,940 3 View
Hello Researchers I am looking for the best and efficient in-vitro translation kit for my work. My main objective is to get good results from the kit.
20 October 2021 4,887 1 View
I want to make 5% DMSO solution for my substrate such as Fatty acids and naphthalene to dissolve.
05 April 2019 2,678 6 View
I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.
27 January 2019 7,899 11 View
I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?
11 October 2018 1,888 4 View
NADPH consumption activities were measured in units of nanomoles of NADPH consumed per nanomole of P450 per minute. How to calculate this?
17 September 2018 1,601 0 View
facing problem in cloning.No colony in transformants plate.
20 August 2018 6,396 9 View
I am adding ribosome binding site (GGAG) in primer to direct protein synthesis.Is this affect the protein synthesis.I am using pET28a vector?
27 February 2018 1,847 0 View
i am trying to express recombinant protein in BL21DE3 using pet28a+ vector.Colony pcr and restriction digestion confirms gene presence in vector.During sds no recombinant protein band was seen?
15 January 2018 3,257 8 View
I have been looking for various biophysical characterizations techniques for recombinants proteins?
18 December 2017 8,766 3 View
I have a clone in pET28 vector, and I want to express this gene in Bacillus cereus.Would it work? If no, then what are the different vector I may use for the same?
15 July 2017 3,502 21 View
I want to break cell BL21 (DE3) for protein estimation by sonication method.What are the differnt conditions,buffers used for this methos?
02 June 2017 2,319 1 View
How to identify BL21 vs BL21(DE3) strain?
23 May 2017 4,411 4 View
Do we express our recombinant protein into TOP10 instead of BL21(DE)? Is this affects its protein expression?
19 April 2017 6,683 2 View
Which restriction enzymes requires fast ap treatment to prevent self ligation?
23 March 2017 1,752 7 View
I want to isolate +ve bacterial genomic dna with high conc.I am using SIGMA kit for isolation?
01 January 1970 4,012 5 View
Buffer composition 50mM tris hcl Nacl 150mM Mgcl2 5mM 10%glycerol
01 January 1970 2,561 3 View
In literature, it has been mentioned that Bacillus P450 bind with Palmitic acid. Upon binding with substrate ,there is peak shift from 420 nm to 390 nm. I am facing problem in peak shift as there...
01 January 1970 2,051 0 View
