NADPH consumption activities were measured in units of nanomoles of NADPH consumed per nanomole of P450 per minute.?

Abhilek Kumar Nautiyal @Abhilek-Nautiyal

17 September 2018 0 2K Report

NADPH consumption activities were measured in units of nanomoles of NADPH consumed per nanomole of P450 per minute.

How to calculate this?

Badges
Science topic

Similar topics
Chemistry
Carbon

More Abhilek Kumar Nautiyal's questions See All

Can anyone explain me how to make 5% DMSO solution?

I want to make 5% DMSO solution for my substrate such as Fatty acids and naphthalene to dissolve.

04 April 2019 2,311 6 View

Protocol for extraction and derivitization of fatty acid for GC analyis?

I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.

26 January 2019 7,689 11 View

Himalayan Black Salt?

Its origin, antioxidant property?

23 January 2019 1,526 3 View

How to fuse two independent domain?

I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?

10 October 2018 1,718 4 View

Facing problem in Cloning?

facing problem in cloning.No colony in transformants plate.

19 August 2018 6,226 9 View

How to extract the cytoplasmic content from human cells ?

Dear all, I want only cytoplasmic content from the human cells to test my compounds. Does anyone knows how to do it or can share the protocol for doing it ?

29 June 2018 9,042 2 View

How I can measure the contraction and expansion of graphene nano particles reinforced in Copper matrix composite?

I have sintered Copper matrix composite which is reinforced by graphene, since graphene shows negative thermal expansion upto a certain temperature and copper shows positive thermal expansion...

04 February 2018 7,835 1 View

What is the effect of CuAl2O4, CuMo2S3 and AlB12 compounds on the Tribology of Copper matrix composite?

During XRD i found the peaks of CuAl2O4, CuMo2S3, AlB12 compounds and i want to know the effect of these compounds on tribological properties of Copper matrix composite during dry sliding. Plz...

16 November 2017 307 2 View

How we can measure the Tensile Strength of a Pellet?

We have a pellet of size 30 mm diameter and 6 mm. thickness,it is a metal matrix composite, how i am supposed to measure the tensile strength of it, by which machine or facility or method it can...

18 July 2017 7,362 4 View

How to identify BL21 vs BL21(DE3) strain?

22 May 2017 4,209 4 View

NADP+/NADPH in bacterial cell lysates?

Hello all, we'd like to pick your brain . The enzyme we're testing requires NADPH to work. We have controls showing that our reaction needs our enzyme and our enzyme definitely needs NADPH. Since...

20 January 2021 232 2 View

Glucose dehydrogenase interference with glucokinase assay?

Hello all, I'm currently working with undergraduate student-researchers (there are no graduate students at my university) to purify and characterize the drug targetability of glucokinase from pig...

25 October 2020 3,553 7 View

Hi, What is the size of NADPH enzyme in Dalton? It's molar mass is 745.4 g/mol?

I am working on a project involved drug metabolism by NADP/NADPH enzyme. I want to remove the enzyme from my reaction mixture. I am looking for the amicon centrifugal concentrator to remove that...

18 November 2019 1,141 3 View

Can tissue samples be deproteinized for measurement of NAPD/NADPH with 4%w/v sulfosalycilic acid?

How will 4% w/v SSA interfere with the NADP/NADPH ratio? Can this method of deproteinization be used as an alternative to 10 kDa cut-off spin columns in order to measure NADP/NADPH ratio?

10 November 2019 3,514 3 View

Hi! Has anyone here needed to quantify the redox state (NAD / NADH and NADP / NADPH ratios) in mutants of Arabidopsis thaliana in spectrophotometer?

I am with a question of quantification by the Promega NAD / NADH Assay Kit kit and NADP / NADPH Assay Kit ... In the Kit there is no standard to make a dilution curve when there is a known...

10 November 2018 768 0 View

In which solution should I dissolve NADPH?

I would like to know in which buffer can I dissolve NADPH used for enzyme activity.It can be potassium or sodium phosphate buffers?

08 September 2018 5,215 0 View

Substitute for beta-NADPH in oxidizing NADPH to NADP+???

Hello, I am trying to conduct a p450 O-deethylation enzyme activity assay, and the protocol calls for B-NADPH as a source to oxidize NAPDH to the non-fluroscant NADP+. B-NADPH is very expensive;...

08 September 2018 2,926 1 View

A sugested procedure of NADPH measurement in oocytes?

There is a paper in Mol Reprod Dev 2015 (Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenetic protein 15 and follicle stimulating hormone....

08 September 2018 514 0 View

Fast and reliable method to determine the NADP/NADPH ratio in yeast cells ?

I want to determine the NADP/NADPH ratio in yeast cells. Any suggestion which method/kit work best with yeast cells. Thanks

06 July 2018 8,877 0 View

Is there a specific excitation wavelength for NADH/NAD+/NADPH/NADP+ in order to observe fluorescece ?

I'd like to be able to detect NADH/NAD+/NADPH/NADP+ by their specific fluoresce.

05 June 2018 5,709 4 View