NADPH consumption activities were measured in units of nanomoles of NADPH consumed per nanomole of P450 per minute.
How to calculate this?
In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?
29 April 2023 2,375 0 View
To overcome this problem Polyether, polyester based, or some other technology will have great result in curing properties. Please help me in detail.
19 April 2023 2,721 0 View
Can anybody please share the Glycerol and Sucrose estimation protocol? I want to check the Glycerol and Sucrose concentration in the yeast culture. I really appreciate any help you can provide.
26 December 2022 7,982 4 View
Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ? There are many solvents, but why only phenol-chloroform is used...
04 December 2022 4,185 2 View
Hello everyone I am looking for a PAGE gel protocol/recipe for RNA.
06 April 2022 2,347 2 View
I am working on the development of a PMMS model. To select the best-performing tools and models, several models are needed to be developed and validated. Can this be replaced by some optimization...
04 April 2022 1,929 5 View
Hello Researchers I need to measure or calculate milliarbitrary units (mAU) of RNAse? A total of 3.8 mAU of RNAse (Life Technologies) per μg of RNA was added to the formulations Can anybody please...
23 March 2022 494 1 View
Anyone please suggest the NABL lab for saffron quality testing in India
03 February 2022 7,447 0 View
Any international body that provide environment pollution data !!
03 January 2022 6,722 2 View
CIPS- Cross Sectionally augmented lm, Pesaran, and Shin unit root test) CADF- Cross Sectional augmented Dickey Fuller unit root test Both CIPS and CADF tests are performed under the null...
01 January 2022 7,362 0 View
Hi guys, I'm stuck on my research, can someone help me to explain how to dock the ligand with a cofactor NADPH using autodocktools ?
25 April 2024 6,269 4 View
Hi, I have been analyzing tot glutathione peroxidase (GPx) activity in mussel cells using cumene hydroperoxide (microplates) and I am a bit unsure how to read/calculate the results. I run a...
03 April 2024 9,344 0 View
I want to prepare reduced form of NADPH. I am using 100mM of Tris (pH8.0) to make the buffer but the absorbance is showing to be 1.6 at 340nm which should supposed to be 0.6. can someone please...
11 March 2024 4,629 3 View
We used to use NADPH as the gene used for nornalization in rat liver experiment. However, NADHP ( and beta actin as well?) may be altered in the setting of fatty liver related experiment. We use...
25 November 2023 2,019 1 View
Here is the question I want to know: With freezing stress, zeaxanthin will synthesis in the dark. Obviously, the reason of the accumulation of Z could be divided in two ways, one is the...
18 June 2023 7,235 0 View
Dear Scientists, I'm in deep. Need for a detailed Protocol for the activity of Glutathion Reductase in insects. It would be great in stored products pests insects. We have a protocol. Without the...
26 February 2023 8,887 0 View
When I used Coot to refine a dimer protein structure, I got a problem. The chain A of dimer protein possesses whole density for cofactor NADP+. But the NADP+ in chain B misses the density in...
22 January 2023 3,764 2 View
Hi, I want to prepare 200 uM NADP solution using NADP Na2;Triphosphopyridine nucleotide disodium salt (Brand: Solarbio, Molecular formula: C21H26N7Na2O17P3, MW: 787.4 g/mol). May I know what...
04 December 2022 8,148 3 View
Hi i am doing so reserch on measuring neuron activity( action and rest potential) . I need your help to find best neuron cell line that has NADPH receptor and can form axon. I worked with HT-22...
28 November 2022 6,876 2 View