Can anyone explain me how to make 5% DMSO solution?

I want to make 5% DMSO solution for my substrate such as Fatty acids and naphthalene to dissolve.

04 April 2019 2,311 6 View

Protocol for extraction and derivitization of fatty acid for GC analyis?

I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.

26 January 2019 7,689 11 View

Himalayan Black Salt?

Its origin, antioxidant property?

23 January 2019 1,526 3 View

How to fuse two independent domain?

I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?

10 October 2018 1,718 4 View

Facing problem in Cloning?

facing problem in cloning.No colony in transformants plate.

19 August 2018 6,226 9 View

How to extract the cytoplasmic content from human cells ?

Dear all, I want only cytoplasmic content from the human cells to test my compounds. Does anyone knows how to do it or can share the protocol for doing it ?

29 June 2018 9,042 2 View

How I can measure the contraction and expansion of graphene nano particles reinforced in Copper matrix composite?

I have sintered Copper matrix composite which is reinforced by graphene, since graphene shows negative thermal expansion upto a certain temperature and copper shows positive thermal expansion...

04 February 2018 7,835 1 View

What is the effect of CuAl2O4, CuMo2S3 and AlB12 compounds on the Tribology of Copper matrix composite?

During XRD i found the peaks of CuAl2O4, CuMo2S3, AlB12 compounds and i want to know the effect of these compounds on tribological properties of Copper matrix composite during dry sliding. Plz...

16 November 2017 307 2 View

How we can measure the Tensile Strength of a Pellet?

We have a pellet of size 30 mm diameter and 6 mm. thickness,it is a metal matrix composite, how i am supposed to measure the tensile strength of it, by which machine or facility or method it can...

18 July 2017 7,362 4 View

How to identify BL21 vs BL21(DE3) strain?

How to identify BL21 vs BL21(DE3) strain?

22 May 2017 4,209 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View