Protocol for cell lysis BL21 (DE3) by sonication?

Abhilek Kumar Nautiyal @Abhilek-Nautiyal

02 June 2017 1 2K Report

I want to break cell BL21 (DE3) for protein estimation by sonication method.What are the differnt conditions,buffers used for this methos?

Abhilek Kumar Nautiyal

I am using 20 sec on nd 10 sec off pulse.Is this ok for my cell

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