In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?
I want to make 5% DMSO solution for my substrate such as Fatty acids and naphthalene to dissolve.
04 April 2019 2,311 6 View
I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.
26 January 2019 7,689 11 View
Its origin, antioxidant property?
23 January 2019 1,526 3 View
I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?
10 October 2018 1,718 4 View
facing problem in cloning.No colony in transformants plate.
19 August 2018 6,226 9 View
Dear all, I want only cytoplasmic content from the human cells to test my compounds. Does anyone knows how to do it or can share the protocol for doing it ?
29 June 2018 9,042 2 View
I have sintered Copper matrix composite which is reinforced by graphene, since graphene shows negative thermal expansion upto a certain temperature and copper shows positive thermal expansion...
04 February 2018 7,835 1 View
During XRD i found the peaks of CuAl2O4, CuMo2S3, AlB12 compounds and i want to know the effect of these compounds on tribological properties of Copper matrix composite during dry sliding. Plz...
16 November 2017 307 2 View
We have a pellet of size 30 mm diameter and 6 mm. thickness,it is a metal matrix composite, how i am supposed to measure the tensile strength of it, by which machine or facility or method it can...
18 July 2017 7,362 4 View
How to identify BL21 vs BL21(DE3) strain?
22 May 2017 4,209 4 View
I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...
01 March 2021 7,131 1 View
I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...
01 March 2021 5,023 5 View
I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...
27 February 2021 4,285 3 View
I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...
26 February 2021 9,580 4 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...
25 February 2021 8,139 1 View
24 February 2021 9,166 3 View
Dear researchers, How can I remove deep eutectic solvents from the medium? You know, generally, deep eutectic solvents consist of ammonium salts and an acid group. Is there any alternative method...
22 February 2021 3,275 3 View
I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...
22 February 2021 1,400 4 View
Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...
21 February 2021 8,132 1 View