How to confirm that NADPH is working fine or not oxidised?

Abhilek Kumar Nautiyal @Abhilek-Nautiyal

30 April 2023 0 2K Report

In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?

Badges
Science topic

Similar topics
Gels

More Abhilek Kumar Nautiyal's questions See All

Does the lower temperature and lower humidity effects the curing time of 2component solvent free adhesive for lamination. If yes then how to resolve?

To overcome this problem Polyether, polyester based, or some other technology will have great result in curing properties. Please help me in detail.

19 April 2023 2,721 0 View

Any protocol for Glycerol and Sucrose estimation?

Can anybody please share the Glycerol and Sucrose estimation protocol? I want to check the Glycerol and Sucrose concentration in the yeast culture. I really appreciate any help you can provide.

26 December 2022 7,982 4 View

Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ?

Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ? There are many solvents, but why only phenol-chloroform is used...

04 December 2022 4,185 2 View

Can anyone please provide me with PAGE gel recipe for RNA ?

Hello everyone I am looking for a PAGE gel protocol/recipe for RNA.

06 April 2022 2,347 2 View

Are there any optimization algorithms which can make a selection of the best-performing model process easy at the initial stages of model selection?

I am working on the development of a PMMS model. To select the best-performing tools and models, several models are needed to be developed and validated. Can this be replaced by some optimization...

04 April 2022 1,929 5 View

How to measure or calculate milliarbitrary units (mAU) of RNAse ?

Hello Researchers I need to measure or calculate milliarbitrary units (mAU) of RNAse? A total of 3.8 mAU of RNAse (Life Technologies) per μg of RNA was added to the formulations Can anybody please...

23 March 2022 494 1 View

Anyone please suggest the NABL lab for saffron quality testing in India other than IIIM Jammu?

Anyone please suggest the NABL lab for saffron quality testing in India

03 February 2022 7,447 0 View

How could I get the Nitrogen and Sulphur dioxide emissions freely available database?

Any international body that provide environment pollution data !!

03 January 2022 6,722 2 View

Which approach is considered to give more reliable results - CIPS or CADF for panel unit root test?

CIPS- Cross Sectionally augmented lm, Pesaran, and Shin unit root test) CADF- Cross Sectional augmented Dickey Fuller unit root test Both CIPS and CADF tests are performed under the null...

01 January 2022 7,362 0 View

What is the Zeta potential of mRNA?

Can anybody brief me on the zeta potential of mRNA?

26 December 2021 8,861 4 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

Methyl jasmonate preparation ?

How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?

01 August 2024 723 0 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

How to prepare polyaniline emeraldine salt ( PANI -ES) sample for Transmission electron microscope (TEM) analysis?

I need to do TEM analysis for my Polyaniline emeraldine salt form. May I know which solvent is appropriate for preparing TEM sample?

25 June 2024 9,086 3 View

Challenges in Stabilizing and Concentrating a Highly Unstable Acetyltransferase for Crystallization?

I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...

19 June 2024 2,590 0 View

How to make 100ppm 1Liter methylene blue stock solution? How much weigh the dye require?

methylene blue stock solution

19 June 2024 10,030 3 View