Can anyone explain me how to make 5% DMSO solution?

I want to make 5% DMSO solution for my substrate such as Fatty acids and naphthalene to dissolve.

04 April 2019 2,311 6 View

Protocol for extraction and derivitization of fatty acid for GC analyis?

I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.

26 January 2019 7,689 11 View

Himalayan Black Salt?

Its origin, antioxidant property?

23 January 2019 1,526 3 View

How to fuse two independent domain?

I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?

10 October 2018 1,718 4 View

Facing problem in Cloning?

facing problem in cloning.No colony in transformants plate.

19 August 2018 6,226 9 View

How to extract the cytoplasmic content from human cells ?

Dear all, I want only cytoplasmic content from the human cells to test my compounds. Does anyone knows how to do it or can share the protocol for doing it ?

29 June 2018 9,042 2 View

How I can measure the contraction and expansion of graphene nano particles reinforced in Copper matrix composite?

I have sintered Copper matrix composite which is reinforced by graphene, since graphene shows negative thermal expansion upto a certain temperature and copper shows positive thermal expansion...

04 February 2018 7,835 1 View

What is the effect of CuAl2O4, CuMo2S3 and AlB12 compounds on the Tribology of Copper matrix composite?

During XRD i found the peaks of CuAl2O4, CuMo2S3, AlB12 compounds and i want to know the effect of these compounds on tribological properties of Copper matrix composite during dry sliding. Plz...

16 November 2017 307 2 View

How we can measure the Tensile Strength of a Pellet?

We have a pellet of size 30 mm diameter and 6 mm. thickness,it is a metal matrix composite, how i am supposed to measure the tensile strength of it, by which machine or facility or method it can...

18 July 2017 7,362 4 View

How to identify BL21 vs BL21(DE3) strain?

How to identify BL21 vs BL21(DE3) strain?

22 May 2017 4,209 4 View

Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

I am looking for PCR primers for bacillus Sp.

15 February 2021 2,552 5 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

Best way for knockout of gene in Bacillus genome?

Hi everyone! I would like to knockout a gene in the genome of Bacillus. To do that I tried to make a construct that has 1) a backbone, 2) a kanamycin resistance cassette, flanked by 3) the...

24 November 2020 489 1 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,043 1 View

How does the spores of Bacillus anthracis cause the anthrax disease and how can it be weaponized?

Anthrax is a disease caused by aerobic spore-forming Bacillus anthracis. This spore can resist environmental changes and resist biological extremes of heat, pH, chemicals, and other factors; it...

20 October 2020 4,658 8 View

Can I transform Bacillus Subtilus with ligation reaction directly?

Hi All, I want to transform bacillus subtilus with ligation reaction directly. Is that possible? To be more clear, I have a shuttling plasmid between bacillus and e.coli. I know usually we need...

14 October 2020 1,743 1 View

I am not able to find the best working protocol for making electrocompetent cells for bacillus sp. Can anyone help me with this?

I am using a protocol for Bacillus subtilis for which there is a requirement for special electroporation media having sorbitol, mannitol and glycerol. I am using puc18 plasmid (conc...

16 July 2020 3,417 2 View