In literature, it has been mentioned that Bacillus P450 bind with Palmitic acid. Upon binding with substrate ,there is peak shift from 420 nm to 390 nm.
I am facing problem in peak shift as there is negligible difference in peak shift.
In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?
29 April 2023 2,375 0 View
To overcome this problem Polyether, polyester based, or some other technology will have great result in curing properties. Please help me in detail.
19 April 2023 2,721 0 View
Can anybody please share the Glycerol and Sucrose estimation protocol? I want to check the Glycerol and Sucrose concentration in the yeast culture. I really appreciate any help you can provide.
26 December 2022 7,982 4 View
Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ? There are many solvents, but why only phenol-chloroform is used...
04 December 2022 4,185 2 View
Hello everyone I am looking for a PAGE gel protocol/recipe for RNA.
06 April 2022 2,347 2 View
I am working on the development of a PMMS model. To select the best-performing tools and models, several models are needed to be developed and validated. Can this be replaced by some optimization...
04 April 2022 1,929 5 View
Hello Researchers I need to measure or calculate milliarbitrary units (mAU) of RNAse? A total of 3.8 mAU of RNAse (Life Technologies) per μg of RNA was added to the formulations Can anybody please...
23 March 2022 494 1 View
Anyone please suggest the NABL lab for saffron quality testing in India
03 February 2022 7,447 0 View
Any international body that provide environment pollution data !!
03 January 2022 6,722 2 View
CIPS- Cross Sectionally augmented lm, Pesaran, and Shin unit root test) CADF- Cross Sectional augmented Dickey Fuller unit root test Both CIPS and CADF tests are performed under the null...
01 January 2022 7,362 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...
30 July 2024 9,824 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...
20 July 2024 7,504 0 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
15 July 2024 2,414 1 View